Isolation of genomic clones homologous to transcribed sequences from human X chromosome
Isolation of genomic clones homologous to transcribed sequences from human X chromosome
复制标题
与人 X 染色体转录序列同源的基因组克隆的分离
DOI：
10.1007/bf01535221
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
L. Shapiro
中科院分区：
文献类型：
--
作者：
P. Yen;B. Marsh;T. Mohandas;L. Shapiro
从人类X染色体文库中分离出了几个与转录序列同源的X染色体DNA克隆。选择这些克隆是因为它们能够在过量未标记的人类重复DNA存在的情况下与32p标记的人类cDNA或与小鼠成纤维细胞cDNA杂交。通过两个标准证明了这些序列的X染色体特异性：当克隆与来自1X和5X细胞的DNA的Southern印迹杂交时，可以看到剂量效应，并且它们与仅含有人类X染色体的小鼠-人杂交细胞的DNA杂交。通过与小鼠cDNA或存在未标记的人类重复序列的人类cDNA杂交，鉴定与cDNA杂交但不与人类重复DNA杂交的限制性内切片段，以及在Northern blots上与poly A+ RNA杂交，检测这些X克隆中转录序列的存在。使用一组携带各种易位的人类X染色体的小鼠-人类体细胞杂交体，将这些克隆映射到人类X染色体上。
Several X chromosome DNA clones homologous to transcribed sequences were isolated from a human X chromosome library. The clones were selected for their ability to hybridize either with32P -labeled human cDNA in the presence of an excess of unlabeled human repetitive DNA or with mouse fibroblast cDNA. The X chromosome specificity of these sequences was demonstrated by two criteria: A dosage effect was seen when the clones were hybridized to Southern blots of DNA from 1X and 5X cells, and they hybridized to DNA from mouse-human hybrid cells containing only the human X chromosome. The presence of transcribed sequences in these X clones was detected by hybridization with mouse cDNA or with human cDNA in the presence of unlabeled human repetitive sequences, by identifying restriction fragments which hybridize with cDNA but not with human repetitive DNA, and by hybridization with poly A+ RNA on Northern blots. These clones were mapped on the human X chromosomes using a panel of mouse-human somatic cell hybrids carrying various translocated human X chromosomes.
Hind II 1.9 kb 重复 DNA 的基因组表示。
影响因子：
14.9
作者：
Manuelidis,L;Biro,PA
通讯作者：
Biro,PA