Significance of circulating tumor cells in patients with squamous cell carcinoma of the head and neck: initial results.
Significance of circulating tumor cells in patients with squamous cell carcinoma of the head and neck: initial results.
复制标题
DOI：
10.1001/archoto.2010.223
复制
发表时间：
2010-12
影响因子：
--
通讯作者：
Chalmers, Jeffrey J.
中科院分区：
文献类型：
--
作者：
Jatana, Kris R.;Balasubramanian, Priya;Lang, Jas C.;Yang, Liying;Jatana, Courtney A.;White, Elisabeth;Agrawal, Amit;Ozer, Enver;Schuller, David E.;Teknos, Theodoros N.;Chalmers, Jeffrey J.
介绍并讨论一种用于分离头颈部癌症患者血液中循环肿瘤细胞（CTC）的高效阴性耗竭方法，并确定CTC的存在与这些患者早期临床结局之间的相关性。对接受手术干预的头颈部鳞状细胞癌（SCCHN）患者进行的前瞻性临床随访研究，这些患者接受了外周血CTC检查。研究人群包括48例诊断为SCCHN并接受手术干预的患者。开发并验证了使用免疫磁性分离从SCCHN患者的血液中分离和定量CTC的阴性耗竭过程。对富集的样品进行细胞角蛋白的免疫染色，以确定从每个患者的血液样品中提取的CTC的数量。CTCs的存在，肿瘤分期，淋巴结状态，临床特征和结果的相关性。无病生存。我们的初始数据，平均随访19.0个月，表明每毫升血液中没有检测到CTC的患者有更高的无病生存概率（P= 0.01）。CTC的存在与年龄、性别、肿瘤部位、分期或淋巴结受累无关。我们的富集技术，基于正常细胞的去除，已用于头颈癌患者的外周血，并收集了随访数据。如果不存在CTC，则在SCCHN中观察到统计学显著改善的无病生存期。具有这种预后能力的血液检测可能对头颈癌患者的治疗具有重要意义。
To present and discuss a high-performance negative depletion method for the isolation of circulating tumor cells (CTCs) in the blood of patients with head and neck cancer and to determine the correlation between the presence of CTCs and early clinical outcome in these patients. Prospective clinical follow-up study of patients with squamous cell carcinoma of the head and neck (SCCHN) undergoing surgical intervention, who had peripheral blood examined for the presence of CTCs. The study population comprised 48 patients diagnosed as having SCCHN and undergoing surgical intervention. A negative depletion process to isolate and quantify CTCs from the blood of patients with SCCHN using immunomagnetic separation was developed and validated. Immunostaining for cytokeratin was performed on the enriched samples to determine the number of CTCs extracted from each patient’s blood sample. Correlation of the presence of CTCs, tumor stage, nodal status, clinical characteristics, and outcome was made. Disease-free survival. Our initial data, that have a mean follow-up of 19.0 months, suggest that patients with no detectable CTCs per milliliter of blood had a significantly higher probability of disease-free survival (P=.01). There was no correlation between the presence of CTCs with regard to age, sex, tumor site, stage, or nodal involvement. Our enrichment technology, based on the removal of normal cells, has been used on the peripheral blood of patients with head and neck cancer for which follow-up data were collected. If no CTCs were present, a statistically significant improved disease-free survival was observed in SCCHN. A blood test with such a prognostic capability could have important implications in the treatment of patients with head and neck cancer.
登录
查看更多内容
影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
11.5
作者：
Riethdorf, Sabine;Fritsche, Herbert;Pantel, Klaus
通讯作者：
Pantel, Klaus
影响因子：
2.8
作者：
Adams, Heiner;Schmid, Patrik;Tzankov, Alexandar
通讯作者：
Tzankov, Alexandar
影响因子：
168.9
作者：
Pantel, K;Izbicki, J;Riethmuller, G
通讯作者：
Riethmuller, G
影响因子：
4.9
作者：
Balasubramanian, Priya;Yang, Liying;Chalmers, Jeffrey J.
通讯作者：
Chalmers, Jeffrey J.