Gelsolin plays a role in the actin polymerization complex of hair cell stereocilia.
Gelsolin plays a role in the actin polymerization complex of hair cell stereocilia.
复制标题
DOI：
10.1371/journal.pone.0011627
复制
发表时间：
2010-07-16
期刊：
影响因子：
3.7
通讯作者：
Brown SD
中科院分区：
文献类型：
--
作者：
Mburu P;Romero MR;Hilton H;Parker A;Townsend S;Kikkawa Y;Brown SD
由PDZ蛋白构建的蛋白质复合物whirlin位于静纤毛尖端，对于静纤毛的发育和伸长至关重要。我们已经证明，在外毛细胞（OHC）中，whirlin是涉及MAGUK蛋白，p55和蛋白4.1R的更大复合物的一部分。Whirlin与p55相互作用，p55仅在构成静纤毛束的长静纤毛中的外毛细胞（OHC）中表达，以及周围最终会退化的较短微绒毛。在红细胞中，p55与蛋白4.1R和血型糖蛋白C形成三方复合物，促进肌动蛋白丝的组装，并且whirlin与p55的相互作用表明其在OHC静纤毛中起类似的作用。然而，在静纤毛中直接参与肌动蛋白丝调节的组分是未知的。我们已经调查了其他组件的whirlin相互作用组通过确定相互作用的合作伙伴p55。我们表明，肌动蛋白帽和切断蛋白，凝溶胶蛋白，是一个旋转复杂的一部分。在OHC中检测到凝溶胶蛋白，它定位于静纤毛较短行的尖端，但不定位于最长行的尖端，并且顶端毛细胞表面的定位模式与p55惊人相似。像p55一样，凝溶胶蛋白在whirler和shaker2突变体中被消融。此外，在凝溶胶蛋白突变体中，耳蜗顶端的静纤毛变得长且散乱，表明静纤毛伸长的调节缺陷。凝溶胶蛋白的鉴定首次提供了参与静纤毛伸长的旋转蛋白支架蛋白复合物与已知肌动蛋白调节分子之间的联系。
A complex of proteins scaffolded by the PDZ protein, whirlin, reside at the stereocilia tip and are critical for stereocilia development and elongation. We have shown that in outer hair cells (OHCs) whirlin is part of a larger complex involving the MAGUK protein, p55, and protein 4.1R. Whirlin interacts with p55 which is expressed exclusively in outer hair cells (OHC) in both the long stereocilia that make up the stereocilia bundle proper as well as surrounding shorter microvilli that will eventually regress. In erythrocytes, p55 forms a tripartite complex with protein 4.1R and glycophorin C promoting the assembly of actin filaments and the interaction of whirlin with p55 indicates that it plays a similar role in OHC stereocilia. However, the components directly involved in actin filament regulation in stereocilia are unknown. We have investigated additional components of the whirlin interactome by identifying interacting partners to p55. We show that the actin capping and severing protein, gelsolin, is a part of the whirlin complex. Gelsolin is detected in OHC where it localizes to the tips of the shorter rows but not to the longest row of stereocilia and the pattern of localisation at the apical hair cell surface is strikingly similar to p55. Like p55, gelsolin is ablated in the whirler and shaker2 mutants. Moreover, in a gelsolin mutant, stereocilia in the apex of the cochlea become long and straggly indicating defects in the regulation of stereocilia elongation. The identification of gelsolin provides for the first time a link between the whirlin scaffolding protein complex involved in stereocilia elongation and a known actin regulatory molecule.
登录
查看更多内容
DOI：
10.1083/jcb.136.3.669
发表时间：
1997-02-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim E;Naisbitt S;Hsueh YP;Rao A;Rothschild A;Craig AM;Sheng M
通讯作者：
Sheng M
DOI：
10.1523/jneurosci.2782-09.2009
发表时间：
2009-12-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Peng AW;Belyantseva IA;Hsu PD;Friedman TB;Heller S
通讯作者：
Heller S
影响因子：
2.8
作者：
FURNESS, DN;RICHARDSON, GP;RUSSELL, IJ
通讯作者：
RUSSELL, IJ
影响因子：
64.5
作者：
WITKE, W;SHARPE, AH;KWIATKOWSKI, DJ
通讯作者：
KWIATKOWSKI, DJ
影响因子：
30.8
作者：
Mburu, P;Mustapha, M;Brown, SDM
通讯作者：
Brown, SDM