The AP2/ERF Gene Family in Triticum durum: Genome-Wide Identification and Expression Analysis under Drought and Salinity Stresses.
The AP2/ERF Gene Family in Triticum durum: Genome-Wide Identification and Expression Analysis under Drought and Salinity Stresses.
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DOI：
10.3390/genes11121464
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发表时间：
2020-12-07
期刊：
影响因子：
3.5
通讯作者：
Heidari P
中科院分区：
文献类型：
--
作者：
Faraji S;Filiz E;Kazemitabar SK;Vannozzi A;Palumbo F;Barcaccia G;Heidari P
AP2/ERF转录因子家族的成员在植物发育、关键代谢产物的生物合成和逆境反应中发挥着关键作用。对硬粒小麦TtAP2s/ERF进行了详细的研究。Dolum)基因组，鉴定出分布在染色体1A-7B上的271个基因。6A染色体携带27个基因，TtAP2s/ERF数目最多。此外，TtAP2s/ERF的重复分析表明，70对重复的基因经过了纯化选择。根据RNA-seq分析，在所有组织中的最高表达水平以及对刺激的反应与DRF和ERF亚家族基因相关。此外，结果表明，TtAP2/ERF基因具有组织特异性表达模式，大部分TtAP2/ERF基因在根组织中显著诱导表达。此外，还选择了13个TtAP2/ERF基因(6个ERF、3个DREB、2个DRF、1个AP2和1个RAV)，通过qRT-PCR进一步分析它们在应对干旱和盐分胁迫方面的潜力。属于DREB亚家族的TtAP2/ERF基因在干旱胁迫和盐胁迫条件下都有明显的诱导。此外，对接模拟揭示了与胁迫反应相关的蛋白质口袋部位的几个残基，这可能有助于未来的定点突变研究，以提高硬粒小麦的逆境耐受性。本研究为进一步研究硬粒小麦这一重要基因家族的进化和功能提供了有价值的启示。
Members of the AP2/ERF transcription factor family play critical roles in plant development, biosynthesis of key metabolites, and stress response. A detailed study was performed to identify TtAP2s/ERFs in the durum wheat (Triticum turgidum ssp. durum) genome, which resulted in the identification of 271 genes distributed on chromosomes 1A-7B. By carrying 27 genes, chromosome 6A had the highest number of TtAP2s/ERFs. Furthermore, a duplication assay of TtAP2s/ERFs demonstrated that 70 duplicated gene pairs had undergone purifying selection. According to RNA-seq analysis, the highest expression levels in all tissues and in response to stimuli were associated with DRF and ERF subfamily genes. In addition, the results revealed that TtAP2/ERF genes have tissue-specific expression patterns, and most TtAP2/ERF genes were significantly induced in the root tissue. Additionally, 13 TtAP2/ERF genes (six ERFs, three DREBs, two DRFs, one AP2, and one RAV) were selected for further analysis via qRT-PCR of their potential in coping with drought and salinity stresses. The TtAP2/ERF genes belonging to the DREB subfamily were markedly induced under both drought-stress and salinity-stress conditions. Furthermore, docking simulations revealed several residues in the pocket sites of the proteins associated with the stress response, which may be useful in future site-directed mutagenesis studies to increase the stress tolerance of durum wheat. This study could provide valuable insights for further evolutionary and functional assays of this important gene family in durum wheat.
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