An Oral-mucosa-on-a-chip sensitively evaluates cell responses to dental monomers.
An Oral-mucosa-on-a-chip sensitively evaluates cell responses to dental monomers.
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DOI：
10.1007/s10544-021-00543-6
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发表时间：
2021-01-11
影响因子：
2.8
通讯作者：
Luo X
中科院分区：
文献类型：
--
作者：
Ly KL;Rooholghodos SA;Rahimi C;Rahimi B;Bienek DR;Kaufman G;Raub CB;Luo X
了解人类牙龈细胞对牙科单体的反应对于开发新型牙科材料至关重要。测试标准的制定旨在为评估牙科材料和器械的生物功能提供指导。然而，传统测试平台的一个缺点是它们不能概括牙龈的多层结构，因此无法评估特定层的细胞反应。先前开发了具有两个细胞层的口腔粘膜芯片作为评估口腔粘膜对牙科生物材料反应的替代平台。粘膜芯片由顶端角质形成细胞层组成，该层通过三微通道网络中的互连孔附着在成纤维细胞嵌入的胶原水凝胶上。在这里，针对甲基丙烯酸 2-羟乙酯 (HEMA) 评估了粘膜芯片中的细胞反应，HEMA 是修复和美容牙科中使用的常见单体。通过将芯片暴露于浓度范围为 1.56 至 25 mM 的 HEMA 中，并与传统孔板单一培养中的细胞进行比较，评估粘膜细胞活力的反应。然后对共培养的细胞进行染色并用落射荧光和共聚焦显微镜成像，以确定对处理的层特异性反应。粘膜芯片被证明比孔板培养物更能敏感地评估 HEMA 改变的细胞活力，特别是在较低剂量（1.56 和 6.25 mM）下。研究结果表明，粘膜芯片通过提供多层组织几何形状、可访问的层特异性信息以及检测细胞反应的更高灵敏度，是测试各种生物材料的传统平台或检测方法的有前途的替代品。
Knowledge of human gingival cell responses to dental monomers is critical for the development of new dental materials. Testing standards have been developed to provide guidelines to evaluate biological functionality of dental materials and devices. However, one shortcoming of the traditional testing platforms is that they do not recapitulate the multi-layered configuration of gingiva, and thus cannot evaluate the layer-specific cellular responses. An oral mucosa-chip with two cell layers was previously developed as an alternative platform to assess the oral mucosa responses to dental biomaterials. The mucosa-chip consists of an apical keratinocyte layer attached to a fibroblast-embedded collagen hydrogel through interconnecting pores in a three-microchannel network. Here, cell responses in the mucosa-chip were evaluated against 2-hydroxyethyl methacrylate (HEMA), a common monomer used in restorative and aesthetic dentistry. The response of mucosal cell viability was evaluated by exposing the chip to HEMA of concentrations ranging from 1.56 to 25 mM and compared to cells in conventional well-plate monoculture. The co-cultured cells were then stained and imaged with epifluorescence and confocal microscopy to determine the layer-specific responses to the treatment. Mucosa-chips were demonstrated to be more sensitive to assess HEMA-altered cell viability than well-plate cultures, especially at lower doses (1.56 and 6.25 mM). The findings suggest that the mucosa-chip is a promising alternative to traditional platforms or assays to test a variety of biomaterials by offering a multi-layered tissue geometry, accessible layer-specific information, and higher sensitivity in detecting cellular responses.
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影响因子：
4.8
作者：
Bienek DR;Frukhtbeyn SA;Giuseppetti AA;Okeke UC;Skrtic D
通讯作者：
Skrtic D
DOI：
10.1021/acs.langmuir.0c01894
发表时间：
2020-09-22
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Hu P;Rooholghodos SA;Pham LH;Ly KL;Luo X
通讯作者：
Luo X
影响因子：
2.8
作者：
Szczepanska, Joanna;Poplawski, Tomasz;Blasiak, Janusz
通讯作者：
Blasiak, Janusz
影响因子：
1.1
作者：
Cho KH;Yu SK;Lee MH;Lee DS;Kim HJ
通讯作者：
Kim HJ
DOI：
10.1016/b978-0-12-397157-9.00077-1
发表时间：
2015-01-01
期刊：
STEM CELL BIOLOGY AND TISSUE ENGINEERING IN DENTAL SCIENCES
影响因子：
--
作者：
Izumi, Kenji;Kato, Hiroko;Feinberg, Stephen E.
通讯作者：
Feinberg, Stephen E.