Genome-Wide Development of MicroRNA-Based SSR Markers in Medicago truncatula with Their Transferability Analysis and Utilization in Related Legume Species.
Genome-Wide Development of MicroRNA-Based SSR Markers in Medicago truncatula with Their Transferability Analysis and Utilization in Related Legume Species.
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蒺藜苜蓿基于 MicroRNA 的 SSR 标记的全基因组开发及其在相关豆科物种中的可转移性分析和利用
DOI：
10.3390/ijms18112440
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发表时间：
2017-11-18
影响因子：
5.6
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Min X;Zhang Z;Liu Y;Wei X;Liu Z;Wang Y;Liu W
微卫星标记是分子标记辅助育种中应用最广泛的标记之一。作为一类功能性标记，基于微RNA的SSR（miRNA-SSR）标记主要在动物中开发，但在植物中的开发和鉴定仍然有限。利用miRNA-SSR标记对蒺藜苜蓿（Medicago truncatula（M. truncatula），并对其在6种豆科植物中的跨种转移性进行了评价。从130个引物中成功设计了169对引物。结果表明，在10个蒺藜属miRNA基因中，以单核苷酸重复序列为主（70.41%），其次为二核苷酸重复序列（14.20%）、复合重复序列（11.24%）和三核苷酸重复序列（4.14%）。对含SSR的miRNA基因的功能分类表明，所有靶点可分为3个基因本体论（GO）类别：17个在生物过程中，11个在分子功能中，14个在细胞组分中。miRNA-SSR标记在其他6种豆科植物中的通用性较高，在74.56%~ 90.53%之间。利用25个Mt-miRNA-SSR标记对20份紫花苜蓿材料进行多态性分析，多态信息含量（PIC）值在0.39 ~ 0.89之间，平均值为0.71，每个标记的等位基因数在3 ~ 18之间，平均值为7.88，具有较高的信息量。本研究是首次对M.这些新的分子标记在豆科植物的遗传多样性分析、分子标记辅助选择和基因分型等方面具有重要的应用价值。
Microsatellite (simple sequence repeats, SSRs) marker is one of the most widely used markers in marker-assisted breeding. As one type of functional markers, MicroRNA-based SSR (miRNA-SSR) markers have been exploited mainly in animals, but the development and characterization of miRNA-SSR markers in plants are still limited. In the present study, miRNA-SSR markers for Medicago truncatula (M. truncatula) were developed and their cross-species transferability in six leguminous species was evaluated. A total of 169 primer pairs were successfully designed from 130 M. truncatula miRNA genes, the majority of which were mononucleotide repeats (70.41%), followed by dinucleotide repeats (14.20%), compound repeats (11.24%) and trinucleotide repeats (4.14%). Functional classification of SSR-containing miRNA genes showed that all targets could be grouped into three Gene Ontology (GO) categories: 17 in biological process, 11 in molecular function, and 14 in cellular component. The miRNA-SSR markers showed high transferability in other six leguminous species, ranged from 74.56% to 90.53%. Furthermore, 25 Mt-miRNA-SSR markers were used to evaluate polymorphisms in 20 alfalfa accessions, and the polymorphism information content (PIC) values ranged from 0.39 to 0.89 with an average of 0.71, the allele number per marker varied from 3 to 18 with an average of 7.88, indicating a high level of informativeness. The present study is the first time developed and characterized of M. truncatula miRNA-SSRs and demonstrated their utility in transferability, these novel markers will be valuable for genetic diversity analysis, marker-assisted selection and genotyping in leguminous species.
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影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
DOI：
10.3390/molecules20058759
发表时间：
2015-05-15
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Liu W;Jia X;Liu Z;Zhang Z;Wang Y;Liu Z;Xie W
通讯作者：
Xie W
基于 MicroRNA 的分子标记：一种新型的基于 PCR 的芸苔属基因分型技术
影响因子：
2
作者：
Fu, Donghui;Ma, Bi;An, Zeshan
通讯作者：
An, Zeshan
影响因子：
4.3
作者：
Trindade, Ines;Capitao, Claudio;dos Santos, Dulce Metelo
通讯作者：
dos Santos, Dulce Metelo