A bioinformatic assay for pluripotency in human cells.

A bioinformatic assay for pluripotency in human cells.
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DOI:
10.1038/nmeth.1580
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发表时间:
2011-04
期刊:
影响因子:
48
通讯作者:
Loring, Jeanne F.
Loring, Jeanne F.
中科院分区:
生物学1区
文献类型:
--
作者:
Mueller, Franz-Josef;Schuldt, Bernhard M.;Williams, Roy;Mason, Dylan;Altun, Gulsah;Papapetrou, Eirini P.;Danner, Sandra;Goldmann, Johanna E.;Herbst, Arne;Schmidt, Nils O.;Aldenhoff, Josef B.;Laurent, Louise C.;Loring, Jeanne F.

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多能干细胞(PSCs)的定义是由它们产生生物体的所有细胞类型的潜力。小鼠PSCs多能性的标准测试是通过生殖系的细胞传播,但对于人类PSCs,研究人员依赖于间接方法,如在免疫缺陷小鼠分化为畸胎瘤。在这里,我们报告了PluriTest,这是一种基于人类细胞基因表达谱的强大的开放访问的生物信息学多能性分析。
Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.
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