Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila.
Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila.
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环腺内保幼激素和 20-羟基蜕皮激素的拮抗作用决定果蝇的发育转变
DOI：
10.1073/pnas.1716897115
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发表时间：
2018-01-02
影响因子：
11.1
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Liu S;Li K;Gao Y;Liu X;Chen W;Ge W;Feng Q;Palli SR;Li S
在脊椎动物中，类固醇激素调节从幼年到成年的发育过渡。昆虫甾体激素20-羟基蜕皮激素（20 E）与保幼激素（JH）协同调控变态，但其确切的相互作用机制尚不清楚。在这里，我们报告说，JH和20 E拮抗对方的生物合成中的一个主要内分泌器官的果蝇幼虫：JH抑制蜕皮激素的生物合成，抑制变态，而20 E抑制JH的生物合成，促进变态。这些数据回答了一个长期存在的问题，即两种主要昆虫激素之间的相互拮抗作用如何调节变态，并可能有助于理解哺乳动物发育过渡的激素调节。在脊椎动物和昆虫中，从幼年期到成年期的发育过渡都是由类固醇激素调节的。在昆虫中，类固醇激素，20-羟基蜕皮激素（20 E），elastinate变态，从而促进这一转变，而倍半萜类保幼激素（JH）拮抗20 E信号，以防止早熟变态在幼虫阶段。然而，人们对这两种激素之间的相互作用机制知之甚少。在这项研究中，我们发现在果蝇幼虫的环腺（RG）中，JH和20 E相互控制对方的生物合成。JH诱导前胸腺（PG）中Krüppel样转录因子基因Kr-h1的表达，前胸腺是RG的一部分，可产生20 E前体蜕皮激素。通过减少类固醇生成的自动调节和PG的大小，高水平的Kr-h1在PG抑制蜕皮激素的生物合成，从而保持少年状态。JH的生物合成被20 E阻止在咽侧体中，该咽侧体是产生JH的RG的另一部分，以确保变态的发生。因此，RG内JH和20 E的拮抗作用决定了果蝇的发育转变。我们的研究提出了在昆虫变态的调节中两种主要激素之间的串扰机制。
Significance In vertebrates, steroid hormones regulate developmental transition from juveniles to adults. Insect steroid hormone, 20-hydroxyecdysone (20E), coordinates with juvenile hormone (JH) to regulate metamorphosis; however, the precise cross-talk mechanism is not well understood. Here, we report that JH and 20E antagonize each other’s biosynthesis in a major endocrine organ of Drosophila larvae: JH suppresses ecdysone biosynthesis and inhibits metamorphosis, whereas 20E suppresses JH biosynthesis and promotes metamorphosis. These data answer a long-standing question on how the mutual antagonism between the two major insect hormones regulates metamorphosis and may help to understand the hormonal regulation of developmental transition in mammals. In both vertebrates and insects, developmental transition from the juvenile stage to adulthood is regulated by steroid hormones. In insects, the steroid hormone, 20-hydroxyecdysone (20E), elicits metamorphosis, thus promoting this transition, while the sesquiterpenoid juvenile hormone (JH) antagonizes 20E signaling to prevent precocious metamorphosis during the larval stages. However, not much is known about the mechanisms involved in cross-talk between these two hormones. In this study, we discovered that in the ring gland (RG) of Drosophila larvae, JH and 20E control each other’s biosynthesis. JH induces expression of a Krüppel-like transcription factor gene Kr-h1 in the prothoracic gland (PG), a portion of the RG that produces the 20E precursor ecdysone. By reducing both steroidogenesis autoregulation and PG size, high levels of Kr-h1 in the PG inhibit ecdysteriod biosynthesis, thus maintaining juvenile status. JH biosynthesis is prevented by 20E in the corpus allatum, the other portion of the RG that produces JH, to ensure the occurrence of metamorphosis. Hence, antagonistic actions of JH and 20E within the RG determine developmental transitions in Drosophila. Our study proposes a mechanism of cross-talk between the two major hormones in the regulation of insect metamorphosis.
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DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
4.6
作者：
Liu, Ying;Sheng, Zhentao;Li, Sheng
通讯作者：
Li, Sheng
影响因子：
4.6
作者：
Huang, Jianhua;Tian, Ling;Wang, Jian
通讯作者：
Wang, Jian
影响因子：
5.3
作者：
Jindra, Marek;Belles, Xavier;Shinoda, Tetsuro
通讯作者：
Shinoda, Tetsuro
影响因子：
4.5
作者：
Jindra M;Uhlirova M;Charles JP;Smykal V;Hill RJ
通讯作者：
Hill RJ