Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells.
Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells.
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miR-27a的下调可能会抑制胃癌细胞的增殖和耐药性。
DOI：
10.1186/1756-9966-30-55
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发表时间：
2011-05-13
期刊：
影响因子：
--
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Zhao X;Yang L;Hu J
本研究旨在首次探讨miR-27 a在胃癌细胞增殖和多药耐药中的作用。采用MTT法、软琼脂法、流式细胞仪法、裸鼠法、real-time PCR、western blot和报告基因法等方法检测miR-27 a在胃癌细胞中的作用。miR-27 a的下调还可以赋予胃癌细胞对药物的敏感性，并可能增加阿霉素在胃癌细胞中的积聚并减少阿霉素的释放量。下调miR-27 a可显著降低P-糖蛋白的表达和cyclin D1的转录活性，上调p21的表达。miR-27 a可能在胃癌的发生、发展和耐药中起重要作用。miR-27 a可能被认为是一个有用的肿瘤治疗靶点。
Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells. The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc. Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21. MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy.
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影响因子：
5.5
作者：
Allen, David L.;Loh, Amanda S.
通讯作者：
Loh, Amanda S.
影响因子：
4.7
作者：
Li, Zhimin;Hu, Sha;Wang, Zehua
通讯作者：
Wang, Zehua
DOI：
10.1016/j.bbrc.2010.01.012
发表时间：
2010-02-12
影响因子：
3.1
作者：
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通讯作者：
Kim, Jae Bum
影响因子：
5.3
作者：
Nishi, Hitoo;Ono, Koh;Kimura, Takeshi
通讯作者：
Kimura, Takeshi
影响因子：
9.7
作者：
Ma, Yihui;Yu, Shuangni;Chen, Jie
通讯作者：
Chen, Jie