AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells.
AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells.
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AGC激酶和MAB 4/MEL蛋白通过限制植物细胞中的侧向扩散来维持PIN极性。
DOI：
10.1016/j.cub.2021.02.028
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发表时间：
2021-05-10
期刊：
影响因子：
--
通讯作者：
Friml J
中科院分区：
文献类型：
--
作者：
Glanc M;Van Gelderen K;Hoermayer L;Tan S;Naramoto S;Zhang X;Domjan D;Včelařová L;Hauschild R;Johnson A;de Koning E;van Dop M;Rademacher E;Janson S;Wei X;Molnár G;Fendrych M;De Rybel B;Offringa R;Friml J
植物激素生长素PIN导出子的极亚细胞定位是生长素定向细胞间运输的关键决定因素，因此是植物细胞和发育生物学的中心课题。缺乏PID(一种磷酸化PIN的激酶)或分子功能未知的MAB4/MEL蛋白的拟南芥突变体显示PIN极性缺陷和表型PIN突变体，但缺乏对这些因素如何传递PIN极性的机械性见解。在这里，通过结合蛋白质生物化学和定量活细胞成像，我们证明了PIN、MAB4/MEL和AGC激酶在质膜上的同一复合体中相互作用。MAB4/MEL被PIN募集到质膜上，并与AGC激酶协同作用，通过限制基于侧向扩散的PIN从极域逃逸来维持PIN的极性。由于AGC激酶介导的PIN磷酸化和MAB4/MEL募集之间的正反馈，PIN-MAB4/MEL-PID蛋白复合体具有自增强特性。从而揭示了AGC蛋白和MAB4/MEL蛋白调控PIN定位和植物发育的分子机制。MAB4/MEL蛋白通过PIN被募集到质膜上，MAB4/MEL和AGC激酶在多蛋白复合体中直接相互作用，PIN磷酸化和MAB4/MEL募集形成一个正反馈环MAB4/MEL，AGC激酶通过限制PIN的横向扩散来维持PIN的极性。结果表明，PIN生长素转运蛋白、AGC激酶和MAB4/MEL蛋白在质膜上形成一个复合体。由于PIN磷酸化和MAB4/MEL募集之间的正反馈，该复合体具有自增强特性，并通过限制PIN的横向扩散而有助于维持PIN的极性。
Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development. MAB4/MEL proteins are recruited to the plasma membrane by PINs PINs, MAB4/MELs, and AGC kinases directly interact in a multiprotein complex PIN phosphorylation and MAB4/MEL recruitment form a positive feedback loop MAB4/MELs and AGC kinases maintain PIN polarity by limiting PIN lateral diffusion Glanc, Van Gelderen et al. show that PIN auxin transporters, AGC kinases, and MAB4/MEL proteins form a complex at the plasma membrane. This complex has self-reinforcing properties thanks to positive feedback between PIN phosphorylation and MAB4/MEL recruitment, and contributes to PIN polarity maintenance by limiting PIN lateral diffusion.
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影响因子：
4.6
作者：
Furutani, Masahiko;Sakamoto, Norihito;Tasaka, Masao
通讯作者：
Tasaka, Masao
影响因子：
21.3
作者：
Ding, Zhaojun;Galvan-Ampudia, Carlos S.;Friml, Jiri
通讯作者：
Friml, Jiri
影响因子：
48
作者：
de Chaumont, Fabrice;Dallongeville, Stephane;Olivo-Marin, Jean-Christophe
通讯作者：
Olivo-Marin, Jean-Christophe
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0910772107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Alassimone, Julien;Naseer, Sadaf;Geldner, Niko
通讯作者：
Geldner, Niko