The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis.
The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis.
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DOI：
10.1186/1747-1028-8-3
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发表时间：
2013-04-11
期刊：
影响因子：
2.3
通讯作者：
Nolte J
中科院分区：
文献类型：
--
作者：
Lührig S;Kolb S;Mellies N;Nolte J
已知BTB-kelch家族蛋白参与多种生物学过程，如迁移、细胞骨架排列、细胞形态调节、蛋白泛素化和基因表达。KBTBD8是这个家族的新成员。该基因是在多能干细胞的比较转录组分析中发现的，因此被认为在多能性的调节中起作用。基因序列与蛋白质序列的比较分析表明，该基因在整个进化过程中具有高度的保守性，特别是在BTB、BACK和kelch的特征区域。我们确定了KBTBD8蛋白在非分裂细胞中的高尔基体是亚细胞定位，并表明KBTBD8与α-微管蛋白在有丝分裂细胞的纺锤体上共定位，提示其在细胞增殖中起作用。总之，KBTBD8是BTB-kelch超家族的新成员，位于高尔基体中，并在有丝分裂期间转运到纺锤体。
Proteins of the BTB-kelch family are known to be involved in multiple biological processes such as migration, cytoskeleton arrangement, regulation of cell morphology, protein ubiquitination and gene expression. KBTBD8 is a new member of this family. The gene was found in a comparative transcriptome analysis of pluripotent stem cells and was therefore suggested to play a role in the regulation of pluripotency. Comparative analysis of the gene and protein sequences revealed a high conservation throughout evolution especially in the characteristic domains of BTB, BACK and kelch. We identified the Golgi apparatus as the subcellular localization of the KBTBD8 protein in non-dividing cells and could show that KBTBD8 co-localizes with α-tubulin on the spindle apparatus of mitotic cells suggesting a role in cell proliferation. In conclusion, KBTBD8 is a new member of the BTB-kelch superfamily that is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis.
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影响因子：
64.8
作者：
Pintard, L;Willis, JH;Peter, M
通讯作者：
Peter, M
影响因子：
4.8
作者：
Nacak, TG;Leptien, K;Kroll, J
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DOI：
10.1083/jcb.131.6.1715
发表时间：
1995-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
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影响因子：
4.8
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影响因子：
11.4
作者：
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通讯作者：
TRAVERS, AA