Changes in gene expression of gastric mucosa during therapeutic acid inhibition
Changes in gene expression of gastric mucosa during therapeutic acid inhibition
复制标题
治疗性酸抑制期间胃粘膜基因表达的变化
DOI：
10.1097/meg.0b013e3282f5dc19
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发表时间：
2008
影响因子：
2.1
通讯作者：
A. Sandvik
中科院分区：
文献类型：
--
作者：
K. Nørsett;A. Lægreid;Waclaw Kusnierczyk;M. Langaas;Sonja Ylving;R. Fossmark;S. Myhre;S. Falkmer;H. Waldum;A. Sandvik
背景 强效酸抑制剂的长期治疗是胃食管反流病的常见治疗方法。服用质子泵抑制剂（PPI）会抑制胃壁细胞的质子泵，从而导致严重且持续的胃酸过少。长期高胃泌素血症会增加粘膜厚度和泌酸粘膜中肠嗜铬样细胞密度。目的 本研究的目的是了解这种非常常见的临床干预措施是否会引起胃粘膜基因表达模式的显着变化。方法 7例胃食管反流病患者纳入本研究。在 3 个月的 PPI 埃索美拉唑治疗之前和即将结束时，对粘膜体进行内窥镜活检。结果 微阵列分析鉴定出 186 个差异表达基因。 PPI 治疗期间基因表达水平发生变化的大部分基因与增殖、凋亡和应激反应有关。结论 这项研究鉴定了许多以前未知的受胃酸分泌抑制影响的基因。进一步表征其表达受强酸抑制调节的基因的功能作用可能会给对强酸抑制的生物反应提供新的见解，包括对临床实践中遇到的胃泌素水平适度升高的粘膜反应。
Background Long-term therapy with potent acid inhibitors is a common treatment for gastro-esophageal reflux disease. Administration of proton pump inhibitors (PPIs) causes profound and continuous hypochlorhydria by inhibition of the proton pump in gastric parietal cells. Long-term hypergastrinaemia increases mucosal thickness and enterochromaffin-like cell density in oxyntic mucosa. Objective The aim of this study was to see whether this very common clinical intervention induces significant changes in the gastric mucosal gene expression pattern. Methods Seven patients suffering from gastro-esophageal reflux disease were included in this study. Endoscopic biopsies were taken from the corpus mucosa before and toward the end of a 3-month treatment with the PPI esomeprazole. Results Microarray analysis identified 186 differentially expressed genes. A high proportion of genes with changed gene expression levels during PPI treatment are involved in proliferation, apoptosis, and stress response. Conclusion This study identified many genes that were not previously known to be affected by inhibition of gastric acid secretion. Further characterization of the functional roles of genes whose expression is modulated by potent acid inhibition may give new insight into the biological responses to potent acid inhibition, including the mucosal response to the moderately increased gastrin levels encountered in clinical practice.
影响因子：
29.4
作者：
Que, FG;Gores, GJ
通讯作者：
Gores, GJ