Differences in growth factor response in smooth muscle cells isolated from adult and neonatal rat arteries.
Differences in growth factor response in smooth muscle cells isolated from adult and neonatal rat arteries.
复制标题
从成年和新生大鼠动脉分离的平滑肌细胞中生长因子反应的差异。
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
N. Ringertz
中科院分区：
文献类型：
--
作者：
A. Hultgårdh‐Nilsson;U. Krondahl;V. Querol;N. Ringertz
The development of atherosclerosis includes an abnormal proliferation of smooth muscle cells (SMCs) in the arterial intima. The factors responsible for this process remain to be identified, but earlier studies have suggested that age-related changes in growth-regulatory mechanisms may be involved. In the present study growth-regulatory mechanisms of neonatal and adult rat SMCs have been compared both in early passage and after subcultivation. Neonatal SMCs in early passage were found to have a high rate of spontaneous DNA synthesis and showed little response to stimulation with growth factors. Early passage adult SMCs showed a lower rate of spontaneous DNA synthesis but responded well to exogenous growth factors. There was no difference in the gene or surface expression of receptors for platelet-derived growth factor (PDGF) between neonatal and adult cells, and there was no significant difference in the amount of inositol phosphate formed in the cells after stimulation with PDGF BB. However, there was increased expression of PDGF A chain mRNA in serum-starved neonatal cells as compared to adult serum-starved SMCs. After subcultivation (seven to nine passages) neonatal SMCs started to become senescent, had a low rate of spontaneous DNA synthesis and were more sensitive to growth factor stimulation than in early passage. Adult SMCs did not demonstrate signs of senescence after subcultivation. The results demonstrate marked differences in the mechanisms regulating growth of neonatal and adult rat SMCs and suggest that the increased sensitivity of adult cells to exogenous growth factors and the inability of these cells to become senescent may be important factors in atherogenesis.
体细胞突变在动脉粥样硬化中的作用。
DOI：
--
发表时间：
1990
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
Penn,A
通讯作者：
Penn,A
在丁酸盐诱导的人 HCT-116 结肠肿瘤细胞克隆分化过程中，细胞表面 EGF 受体表达增加。
影响因子：
3.7
作者：
Nathan,DF;Burkhart,SR;Morin,MJ
通讯作者：
Morin,MJ
DOI：
10.1089/dna.1983.2.329
发表时间：
1983-01-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
通讯作者：
BAXTER, JD