Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis.
Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis.
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DOI：
10.1016/j.devcel.2008.05.015
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发表时间：
2008-08
影响因子：
11.8
通讯作者：
Bortvin, Alex
中科院分区：
文献类型：
--
作者：
Soper, Sarah F. C.;van der Heijden, Godfried W.;Hardiman, Tara C.;Goodheart, Mary;Martin, Sandra L.;de Boer, Peter;Bortvin, Alex
严格控制转座子活性对于生殖系的完整性至关重要。最近，生殖细胞特异性细胞器nuage被提出在转座子抑制中发挥作用。为了验证这一假设，我们破坏了果蝇nuage蛋白Maelstrom的小鼠同源物。观察了对雄性减数分裂染色体联会和转座因子（TEs）去阻遏的影响。在成年的Mael−/−睾丸中，LINE-1（L1）去阻遏发生在减数分裂开始时。结果，Mael−/−精母细胞充满了L1核糖核蛋白（RNP），这些核糖核蛋白聚集在大的细胞质包体和细胞核中。具有核L1 RNP的Mael−/−精母细胞即使在没有SPO 11产生的减数分裂双链断裂的情况下也表现出大量的DNA损伤和严重的染色体不联会。这项研究表明，MAEL，一个nuage组件，是不可或缺的沉默TE和鉴定减数分裂的启动作为一个重要步骤TE控制在男性生殖系。
Tight control of transposon activity is essential for the integrity of the germline. Recently, a germ cell-specific organelle, nuage, was proposed to play a role in transposon repression. To test this hypothesis, we disrupted a murine homolog of a Drosophila nuage protein Maelstrom. Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs) were observed. In the adult Mael−/− testes, LINE-1 (L1) derepression occurred at the onset of meiosis. As a result, Mael−/− spermatocytes were flooded with L1 ribonucleoproteins (RNPs) that accumulated in large cytoplasmic enclaves and nuclei. Mael−/− spermatocytes with nuclear L1 RNPs exhibited massive DNA damage and severe chromosome asynapsis even in the absence of SPO11-generated meiotic double strand breaks. This study demonstrates that MAEL, a nuage component, is indispensable for the silencing of TEs and identifies the initiation of meiosis as an important step in TE control in the male germline.
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影响因子：
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