Reducing non-specific binding and uptake of nanoparticles and improving cell targeting with an antifouling PEO-b-PgammaMPS copolymer coating.
Reducing non-specific binding and uptake of nanoparticles and improving cell targeting with an antifouling PEO-b-PgammaMPS copolymer coating.
复制标题
DOI：
10.1016/j.biomaterials.2010.03.036
复制
发表时间：
2010-07
期刊：
影响因子：
14
通讯作者：
Mao H
中科院分区：
文献类型：
--
作者：
Chen H;Wang L;Yeh J;Wu X;Cao Z;Wang YA;Zhang M;Yang L;Mao H
One of the major limitations impeding the sensitivity and specificity of biomarker targeted nanoparticles is non-specific binding by biomolecules and uptake by the reticuloendothelial system (RES). We report the development of an antibiofouling polysiloxane containing amphiphilic diblock copolymer, poly(ethylene oxide)-block-poly(γ-methacryloxypropyltrimethoxysilane) (PEO-b-PγMPS), for coating and functionalizing high quality hydrophobic nanocrystals such as iron oxide nanoparticles and quantum dots. These PEO-b-PγMPS coated nanocrystals were colloidally stable in biological medium and showed low non-specific binding by macromolecules after incubation with 100% fetal bovine serum. Both in vitro experiments with macrophages and in vivo biodistribution studies in mice revealed that PEO-b-PγMPS copolymer coated nanocrystals have an antibiofouling effect that reduces non-specific cell and RES uptake. Surface functionalization with amine groups was accomplished through co-crosslinking the polysiloxane coating layer and (3-Aminopropyl) trimethoxysilane in aqueous solution. Tumor integrin αvβ3 targeting peptide cyclo-RGD ligands were conjugated on the nanoparticles through a heterobifunctional linker. The resulting integrin αvβ3 targeting nanoparticle conjugates showed improved cancer cell targeting with a stronger affinity to U87MG glioma cells, which have a high expression of αvβ3 integrins, but minimal binding to MCF-7 (low expression of αvβ3 integrins).
登录
查看更多内容
影响因子：
14
作者：
Jain, Tapan K.;Foy, Susan P.;Erokwu, Bernadette;Dimitrijevic, Sanja;Flask, Christopher A.;Labhasetwar, Vinod
通讯作者：
Labhasetwar, Vinod
影响因子：
3.9
作者：
Bagwe, RP;Hilliard, LR;Tan, WH
通讯作者：
Tan, WH
影响因子：
15
作者：
Lee, Haerim;Yu, Mi Kyung;Jon, Sangyong
通讯作者：
Jon, Sangyong
影响因子：
8.6
作者：
Jana, Nikhil R.;Earhart, Christopher;Ying, Jackie Y.
通讯作者：
Ying, Jackie Y.
影响因子：
15
作者：
Duan, Hongwei;Nie, Shuming
通讯作者：
Nie, Shuming