Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization.
Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization.
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DOI：
10.1093/jxb/ert054
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Okamoto T
中科院分区：
文献类型：
--
作者：
Abiko M;Maeda H;Tamura K;Hara-Nishimura I;Okamoto T
在被子植物中，受精和随后的合子发育发生在深埋于子房的胚囊中；因此，对这些过程的解释很差。在这项研究中，基于微阵列的转录组分析从水稻花中分离的精子细胞、卵细胞和受精卵，以确定参与配子和/或早期合子发育的候选基因。获得细胞类型特异性转录组，鉴定受精后受精卵中上调或下调的基因，以及雄性和雌性配子中富集的基因。共获得合子中325个推定上调基因和94个推定下调基因。有趣的是，一些编码同源盒蛋白或转录因子的基因在受精后被鉴定为高度上调的基因，并且上调基因的基因本体在染色质/DNA组织和组装相关功能中高度富集。由于编码甲基转移酶1的基因被鉴定为受精后受精卵中高度上调的基因，因此监测了该酶抑制剂对受精卵发育的影响。该抑制剂部分影响水稻受精卵的极性或分裂不对称性，但不影响正常胚胎的发生。
In angiosperms, fertilization and subsequent zygotic development occur in embryo sacs deeply embedded in the ovaries; therefore, these processes are poorly elucidated. In this study, microarray-based transcriptome analyses were conducted on rice sperm cells, egg cells, and zygotes isolated from flowers to identify candidate genes involved in gametic and/or early zygotic development. Cell type-specific transcriptomes were obtained, and up- or down-regulated genes in zygotes after fertilization were identified, in addition to genes enriched in male and female gametes. A total of 325 putatively up-regulated and 94 putatively down-regulated genes in zygotes were obtained. Interestingly, several genes encoding homeobox proteins or transcription factors were identified as highly up-regulated genes after fertilization, and the gene ontology for up-regulated genes was highly enriched in functions related to chromatin/DNA organization and assembly. Because a gene encoding methyltransferase 1 was identified as a highly up-regulated gene in zygotes after fertilization, the effect of an inhibitor of this enzyme on zygote development was monitored. The inhibitor appeared partially to affect polarity or division asymmetry in rice zygotes, but it did not block normal embryo generation.
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