Diversity and abundance of bacteria in an underground oil-storage cavity.
Diversity and abundance of bacteria in an underground oil-storage cavity.
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DOI：
10.1186/1471-2180-2-23
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发表时间：
2002-08-28
期刊：
影响因子：
4.2
通讯作者：
Kaku N
中科院分区：
文献类型：
--
作者：
Watanabe K;Kodama Y;Kaku N
近年来，居住在地下油田的微生物引起了人们的广泛关注。由于地下储油腔底部不受地表水污染，可以很容易地获得完整的地下水，因此对地下储油腔的研究有望为了解地下油田的微生物生态提供有价值的信息。从位于日本岩手县Kuji的储油腔中获得的地下水中提取DNA，采用通用引物和细菌特异性引物组合PCR扩增16S rRNA基因（16S rDNA）片段。对154个克隆进行序列分析，得到31种不同的细菌序列类型（序列相似性为bbb98的一个克隆或一组克隆）。主要序列类型与Desulfotomaculum、Acetobacterium、Desulfovibrio、desulfoobacula、Zoogloea和thiommicrospira反硝化菌有关。利用特异性引物对这些主要序列类型所代表的细菌种群在地下水中的丰度进行了定量竞争PCR评估，结果表明，除与desulfobactera相关的rDNA外，5种rDNA类型在细菌总rDNA中所占比例显著（大于1%）。储油腔内的细菌种类之多出乎意料。克隆的16S rDNA序列的系统发育关系表明，具有不同类型能量代谢的细菌在腔内共存。
Microorganisms inhabiting subterranean oil fields have recently attracted much attention. Since intact groundwater can easily be obtained from the bottom of underground oil-storage cavities without contamination by surface water, studies on such oil-storage cavities are expected to provide valuable information to understand microbial ecology of subterranean oil fields. DNA was extracted from the groundwater obtained from an oil-storage cavity situated at Kuji in Iwate, Japan, and 16S rRNA gene (16S rDNA) fragments were amplified by PCR using combinations of universal and Bacteria-specific primers. The sequence analysis of 154 clones produced 31 different bacterial sequence types (a unique clone or group of clones with sequence similarity of > 98). Major sequence types were related to Desulfotomaculum, Acetobacterium, Desulfovibrio, Desulfobacula, Zoogloea and Thiomicrospira denitrificans. The abundance in the groundwater of bacterial populations represented by these major sequence types was assessed by quantitative competitive PCR using specific primers, showing that five rDNA types except for that related to Desulfobacula shared significant proportions (more than 1%) of the total bacterial rDNA. Bacteria inhabiting the oil-storage cavity were unexpectedly diverse. A phylogenetic affiliation of cloned 16S rDNA sequences suggests that bacteria exhibiting different types of energy metabolism coexist in the cavity.
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DOI：
10.1099/00207713-48-1-215
发表时间：
1998-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Brauman, A;M端ller, JA;Schink, B
通讯作者：
Schink, B
影响因子：
3.2
作者：
Cornillot, E;Croux, C;Soucaille, P
通讯作者：
Soucaille, P
影响因子：
4.4
作者：
Lee, SY;Bollinger, J;Ogram, A
通讯作者：
Ogram, A
影响因子：
64.8
作者：
LHARIDON, S;REYSENBACH, AL;JEANTHON, C
通讯作者：
JEANTHON, C
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M