Mechanism of electroacupuncture at acupoints of the lung meridian through PKA/PKC regulation of TRPV1 in chronic cough after lung surgery in guinea pigs.
Mechanism of electroacupuncture at acupoints of the lung meridian through PKA/PKC regulation of TRPV1 in chronic cough after lung surgery in guinea pigs.
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DOI：
10.21037/jtd-23-409
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发表时间：
2023-04-28
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
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作者：
针灸在治疗咳嗽、哮喘、慢性阻塞性肺疾病(COPD)等肺部疾病方面取得了良好的效果，但针灸治疗肺部手术后慢性咳嗽的相关机制尚不清楚。我们探讨了针刺疗法能否通过调节肺手术后慢性咳嗽的瞬时受体电位-香草酸-1(TRPV1)信号通路，来改善肺手术后慢性咳嗽的症状。将豚鼠分为假手术组(Sham)、模型组(Model)、电针+模型组(EA+M)、H89+模型组(H89+M)、Go6983+模型组(Go6983+M)。通过测量咳嗽症状(咳嗽次数/咳嗽潜伏期)作为结果标准来确定治疗效果。采用酶联免疫吸附试验(EL ISA)检测支气管肺泡灌洗液(BALF)和全血中炎性细胞因子的水平。肺组织苏木精-伊红(H&E)染色。Western blotting检测p-PKA、p-PKC和p-TRPV1蛋白的表达。用实时定量聚合酶链式反应(RT-PCR)检测TRPV1、P物质(SP)、降钙素基因相关肽(CGRP)和神经激肽-1R(NK1R)的mRNA水平。针刺可明显减少慢性咳嗽豚鼠肺部手术后的咳嗽次数，延长咳嗽潜伏期。此外，针灸减少了对肺组织的损伤。针刺治疗后，各治疗组炎性细胞因子水平降低，p-PKA、p-PKC、p-TRPV1表达明显抑制，TRPV1、SP、CGRP、NK1R基因表达显著降低。针刺通过PKA/PKC调节TRPV1信号通路，改善肺手术后豚鼠慢性咳嗽。本研究结果表明针刺可能是治疗肺手术后慢性咳嗽的有效方法，并阐明了其可能的作用机制，为临床治疗肺手术后慢性咳嗽提供了理论依据。
Acupuncture has achieved good results in the treatment of cough, asthma, chronic obstructive pulmonary disease (COPD) and other lung diseases, but the mechanism associated with acupuncture in the treatment of chronic cough induced by lung surgery is unknown. We investigated whether acupuncture therapy could improve the symptoms of chronic cough after lung surgery through cyclic-AMp dependent protein kinase A (PKA)/cyclic-AMp dependent protein kinase C (PKC) regulation of the transient receptor potential vanilloid-1 (TRPV1) signaling pathway. The guinea pigs were divided into 5 groups: the Sham operation Group (Sham), the Model Group (Model), the Electroacupuncture + Model Group (EA + M), the H89 + Model Group (H89 + M) and the Go6983 + Model Group (Go6983 + M). The effect of treatment was determined by measuring cough symptoms (number of coughs/cough incubation period) as the outcome criterion. The levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) and blood were determined by enzyme-linked immunosorbent assays (ELISA). Lung tissue was stained with hematoxylin and eosin (H&E). The expression of p-PKA, p-PKC and p-TRPV1 proteins was measured by Western blotting. The mRNA levels of TRPV1, Substance P (SP), calcitonin gene-related peptide (CGRP) and neurokinin-1R (NK1R) were measured by real-time polymerase chain reaction (RT-PCR). Acupuncture significantly reduced the cough frequency and prolonged the cough latency of chronic cough in guinea pigs after lung surgery. In addition, acupuncture reduced the damage to lung tissue. The levels of inflammatory cytokines decreased in all treatment groups, the expression levels of p-PKA, p-PKC and p-TRPV1 were significantly inhibited and the mRNA levels of TRPV1, SP, CGRP and NK1R decreased significantly after acupuncture treatment. Acupuncture therapy ameliorated chronic cough in guinea pigs after lung surgery by regulating the TRPV1 signaling pathway via PKA/PKC. Our results showed that acupuncture may be an effective treatment of chronic cough after lung surgery, and also clarified the potential mechanism, which provides a theoretical basis for the clinical treatment of patients with chronic cough after lung surgery.
影响因子：
3.7
作者：
Gao X;Zhuang J;Zhao L;Wei W;Xu F
通讯作者：
Xu F
影响因子：
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作者：
Tu, Hengjia;Zhang, Qingling
通讯作者：
Zhang, Qingling