Fibulin-4 is associated with tumor progression and a poor prognosis in ovarian carcinomas.
Fibulin-4 is associated with tumor progression and a poor prognosis in ovarian carcinomas.
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纤连蛋白4（Fibulin - 4）与卵巢癌的肿瘤进展及不良预后相关。
DOI：
10.1186/s12885-015-1100-9
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发表时间：
2015-03-04
期刊：
影响因子：
3.8
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen J;Liu Z;Fang S;Fang R;Liu X;Zhao Y;Li X;Huang L;Zhang J
纤维蛋白-4是细胞外糖蛋白纤维蛋白家族的成员，与某些癌症的进展有关。然而，迄今为止还没有关于fibulin-4在卵巢癌进展中的功能的信息。本研究采用免疫组化和真实的时间逆转录聚合酶链反应（RT-PCR）检测了正常卵巢组织、卵巢肿瘤、高侵袭性卵巢癌细胞亚克隆和低侵袭性卵巢癌细胞亚克隆中fibulin-4 mRNA和蛋白的表达。采用酶联免疫吸附法和电化学发光法检测卵巢肿瘤患者血清中fibulin-4、CA-125和CA-19 -9的水平。为了评估fibulin-4的血管生成特性，通过免疫组织化学方法分析了卵巢癌中血管内皮生长因子（VEGF）的表达和肿瘤微血管密度。Fibulin-4在卵巢癌中表达上调，与MVD、VEGF表达呈正相关。Fibulin-4过表达与卵巢癌患者的分期、分化程度、淋巴结转移及预后不良显著相关。卵巢癌患者血清中Fibulin-4、CA-125和CA-19 -9水平明显高于卵巢良性肿瘤和正常对照组。与CA-125和CA-19 -9相比，Fibulin-4具有更好的诊断敏感性和特异性。Fibulin-4是一种新的基因，在卵巢癌中过度表达，并与预后不良的临床病理特征相关。这项研究表明，fibulin-4可能作为一个新的预后因素，并作为一个潜在的治疗目标，卵巢癌患者在未来。
Fibulin-4, a member of the fibulin family of extracellular glycoproteins, is implicated in the progressions of some cancers. However, no information has been available to date regarding the function of fibulin-4 in ovarian carcinoma progression. In this study, fibulin-4 mRNA and protein expression in normal ovarian tissue, ovarian tumor, high invasive subclones and low invasive subclones were evaluated by immunohistochemistry and real time reverse transcriptase-polymerase chain reaction (RT-PCR). The serum levels of fibulin-4, cancer antigen 125 (CA-125) and cerbohydrate antigen 199 (CA19-9) in patients with ovarian tumor were measured by enzyme-linked immunosorbent assay and electrochemiluminescent immunoassay. To assess the angiogenic properties of fibulin-4, vascular endothelial growth factor (VEGF) expression and tumor microvessel density were analyzed in ovarian carcinoma by immunohistochemistry. Fibulin-4 expression was upregulated in ovarian carcinoma, and positively correlated with MVD and VEGF expression. Fibulin-4 overexpression was significantly associated with advanced stage, low differentiation, lymph node metastasis and poor prognosis in patients with ovarian cancer. The serum levels of fibulin-4, CA-125 and CA19-9 in patients with ovarian carcinoma were much higher than those with benign ovarian tumors and normal controls. Compared to CA-125 and CA19-9, fibulin-4 had better diagnostic sensitivity and specificity. Fibulin-4 is a novel gene that is found overexpressed in ovarian cancer and associated with poor prognostic clinicopathologic features. This study shows that fibulin-4 may serve as a new prognostic factor and as a potential therapeutic target for patients with ovarian cancer in the future.
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DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Bardin, A;Moll, F;Pujol, P
通讯作者：
Pujol, P
影响因子：
11.2
作者：
Albig, AR;Neil, JR;Schiemann, WP
通讯作者：
Schiemann, WP
DOI：
10.1007/s00432-008-0526-8
发表时间：
2009-07-01
影响因子：
3.6
作者：
Chen, Jie;Zhang, Jie;Fu, Maosun
通讯作者：
Fu, Maosun
影响因子：
3.5
作者：
Gallagher, WM;Greene, LM;Conseiller, E
通讯作者：
Conseiller, E