Microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus.
Microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus.
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DOI：
10.1016/j.jcv.2009.09.020
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发表时间：
2009-12
影响因子：
8.8
通讯作者：
Walsh, Edward E.
中科院分区：
文献类型：
--
作者：
Falsey, Ann R.;Formica, Maria A.;Walsh, Edward E.
Human metapneumovirus (hMPV) is a newly discovered virus which causes respiratory illness in persons of all ages. A simple and rapid method to determine neutralizing antibody titers against hMPV is needed to facilitate the development of vaccines and therapeutics for hMPV. Therefore, we sought to adapt the methodology used for RSV microneutralization assay (MNA) to measure neutralizing antibody titers against hMPV. Serial 2-fold dilutions of serum were made in 96 well microtiter plates and incubated with ~ 50 pfu of hMPV A or B strain for 60 minutes at room temperature. LLC-MK2 cells were added to the serum-virus mixtures and plates incubated at 35°C in CO2 for 5 days. Plates were fixed with acetone; air dried, blocked and then developed with monoclonal antibody to the hMPV N protein followed by horse radish peroxidase labeled antibody and substrate. Neutralization titer was defined as the titer of serum that reduced color development by 50% compared to the positive control wells. Titers measured by MNA correlated well with those determined by standard plaque reduction assay (R= 0.77). Neutralization titers determined by MNA demonstrated excellent inter-assay variability (coefficient of variance = 7%). In addition, there was good correlation of antibody titers from 10 hMPV infected adults measured by MNA using either group A or group B hMPV (R = 0.87). MNA is a simple and reproducible method for the measurement of serum neutralizing antibody against hMPV
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影响因子：
82.9
作者：
van den Hoogen BG;de Jong JC;Groen J;Kuiken T;de Groot R;Fouchier RA;Osterhaus AD
通讯作者：
Osterhaus AD
影响因子：
5.5
作者：
Falsey, AR;Walsh, EE
通讯作者：
Walsh, EE
影响因子：
5.4
作者：
Williams, JV;Tollefson, SJ;Crowe, JE
通讯作者：
Crowe, JE
影响因子：
11.8
作者：
van den Hoogen BG;Herfst S;Sprong L;Cane PA;Forleo-Neto E;de Swart RL;Osterhaus AD;Fouchier RA
通讯作者：
Fouchier RA
影响因子：
3.1
作者：
Biacchesi, S;Skiadopoulos, MH;Buchholz, UJ
通讯作者：
Buchholz, UJ