Imprinting and expression analysis of a non-coding RNA gene in the mouse Dlk1-Dio3 domain
Imprinting and expression analysis of a non-coding RNA gene in the mouse Dlk1-Dio3 domain
复制标题
小鼠 Dlk1-Dio3 结构域非编码 RNA 基因的印迹和表达分析
DOI：
10.1007/s10735-011-9337-3
复制
发表时间：
2011-06
影响因子：
3.2
通讯作者：
Q. Wu
中科院分区：
文献类型：
--
作者：
F. W. Zhang, T. B. Zeng, Z. B. Han, H. J. He, Y. C;Q. Wu
Dlk 1-Dio 3印迹结构域不仅与胚胎和胎盘的生长发育有关，而且还影响成年人的代谢和脑功能。在这项研究中，我们确定了印迹状态的小鼠非编码RNA基因，B830012 L14 Rik，映射到Dlk 1-Dio 3域的多态性和测序为基础的方法。印迹分析表明，该基因在E15.5，E18.5和出生后1天的小鼠中表达。通过北方印迹检测到约1.9和3.5 kb的两种转录物。此外，我们研究了该基因在小鼠发育过程中的时空表达模式。原位杂交结果显示，B830012 L14 Rik在E13.5和E15.5主要表达于前脑、垂体、脊柱软骨原基、肺和肝脏。实时定量RT-PCR结果显示，B830012 L14 Rik在脑、心、肺和肝中的表达在E15.5高于E12.5和E18.5。此外，从E12.5到E15.5，基因表达在脑中进行性增加，而从E15.5到E19.5则降低。本研究为深入了解Dlk 1-Dio 3印迹簇的印迹、基因组特征和表达调控提供了理论依据。
The Dlk1-Dio3 imprinted domain not only is implicated growth and development of the embryo and placenta, but also affects adult metabolism and brain function. In this study, we identified the imprinting status of a mouse non-coding RNA gene, B830012L14Rik, mapped to the Dlk1-Dio3 domain by the polymorphism- and sequencing-based approach. Imprinting analysis showed that the gene was expressed maternally at E15.5, E18.5 and postnatal day 1 mice. Two transcripts of approximately 1.9 and 3.5 kb were detected by northern blot. Furthermore, we examined the spatiotemporal expression patterns of the gene during the mouse development. In situ hybridization analysis showed that B830012L14Rik was mainly expressed in forebrain, pituitary, cartilage primordium of spinal column, lung and liver at E13.5 and E15.5. The results of real-time quantitative RT-PCR showed that the B830012L14Rik expression in brain, heart, lung and liver was higher at E15.5 than at E12.5 and E18.5. Furthermore, the gene expression increased progressively in brain from E12.5 to E15.5 whereas decreased from E15.5 to E19.5. This study may provide further insights into the imprinting, genomic features and expression regulation of the Dlk1-Dio3 imprinted cluster.
登录
查看更多内容
影响因子：
3.2
作者：
Gao, Mengya;Liu, Qi;Wu, Qiong
通讯作者：
Wu, Qiong
影响因子：
3.2
作者：
Moorman, AFM;Houweling, AC;Christoffels, VM
通讯作者：
Christoffels, VM
影响因子：
7
作者：
Seitz, H;Royo, H;Cavaillé, J
通讯作者：
Cavaillé, J
影响因子：
1.7
作者：
Tevendale, M.;Watkins, M.;Ferguson-Smith, A. C.
通讯作者：
Ferguson-Smith, A. C.
影响因子：
7
作者：
Okamura, K;Hagiwara-Takeuchi, Y;Ito, T
通讯作者：
Ito, T