Inoculation with Arbuscular Mycorrhizal Fungi Reinforces Tea Plant’s Tolerance to Salinity
Inoculation with Arbuscular Mycorrhizal Fungi Reinforces Tea Plant’s Tolerance to Salinity
复制标题
接种丛枝菌根真菌可增强茶树对盐度的耐受性
DOI：
10.1007/s00344-021-10529-6
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发表时间：
2021-11
影响因子：
4.8
通讯作者：
Chunmei Gong
中科院分区：
文献类型：
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作者：
Shasha Guo;Qi Wang;Lei Tang;Tianxin Zhang;Jiayang Li;Yao Xiao;Yuefang Gao;Juan Bai;Bin Xiao;Chunmei Gong
严重限制植物生长和生产力的一个主要胁迫因素是由盐分引起的土壤退化。丛枝菌根真菌（AMF）与大多数农业植物建立共生关系，并在土壤胁迫下促进生长。本研究以盐胁迫下接种AMF的茶树和未接种AMF的茶树为研究对象，利用RNA-seq技术和茶树基因组测序技术，研究了AMF对盐胁迫下茶树生长和品质的影响。在盐胁迫下，AMF处理和不处理的茶树叶片中共检测到1402个和1915个差异表达基因。功能注释分析表明，这些DEG主要参与代谢过程、膜和细胞部分以及催化活性功能。京都基因百科全书和基因组途径分析表明，在盐胁迫下，有AMF存在时，DEG富集在纤维素合成上，无AMF存在时，DEG富集在木质素和纤维素合成上。进一步的实验证实，盐胁迫后，AMF处理的茶树木质部维管组织和厚壁组织中木质素的沉积量增加，木质素的自生能力减弱。接种AMF可缓解盐胁迫，表现在木质素和纤维素积累减少。接种AMF的茶树通过渗透调节和保护酶的作用增强了茶树的耐性。总之，本研究结果为了解AMF在北方茶区适应逆境和保持鲜叶品质方面的作用提供了一个较全面的认识。
A major stress factor that can seriously limit plant growth and productivity is the soil degradation caused by salinity. Arbuscular mycorrhizal fungi (AMF) establish symbioses with most agricultural plants and improve growth under soil stress. In .this study, tea plants with and without AMF inoculation under salt stress were investigated with the RNA-seq and the genome .sequencing of tea plant, obtaining a positive insight into AMF-colonized involved in the improvement of growth and quality .afected by lignin and cellulose synthesis under salt stress. Totals of 1402 and 1915 diferentially expressed genes (DEGs) .were identifed in tea leaf with and without AMF under salt stress. Functional annotation analysis revealed that these DEGs .were mainly involved in metabolic process, membrane and cell part and catalytic activity function. The Kyoto Encyclopedia .of Genes and Genomes pathways analysis showed that the DEGs were enriched on the cellulose synthesis with AMF or .enriched on the lignin and cellulose synthesis without AMF under salt stress. Further experiments verifed that the more .lignin deposited on the xylem vascular and the sclerenchyma of tea plants after salt stress, and lignin autofuorescence in .tea plants with AMF was weaker than that without AMF. AMF inoculation could alleviate the salt stress, which showed in .the decrease of lignin and cellulose accumulation. Moreover, the tea plants inoculated AMF increased tolerance by osmotic .regulation and protective enzymes. In general, our results provide a more comprehensive insight into the role of AMF in .adapting the adverse condition and keep the quality of fresh tea leaves in the northern Tea Area.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
4.4
作者：
Hashem A;Alqarawi AA;Radhakrishnan R;Al-Arjani AF;Aldehaish HA;Egamberdieva D;Abd Allah EF
通讯作者：
Abd Allah EF
影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
影响因子：
4.5
作者：
Adhikari A;Khan MA;Lee KE;Kang SM;Dhungana SK;Bhusal N;Lee IJ
通讯作者：
Lee IJ
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