Involvement of the fas system in hepatitis C virus recurrence after liver transplantation
Involvement of the fas system in hepatitis C virus recurrence after liver transplantation
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fas系统参与肝移植后丙型肝炎病毒复发
DOI：
10.1053/jlts.2000.9742
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发表时间：
2000
影响因子：
4.6
通讯作者：
F. Pons‐Romero
中科院分区：
文献类型：
--
作者：
J. Crespo;Monteserrat Rivero;M. Mayorga;E. Fábrega;F. Casafont;M. Gómez‐Fleitas;F. Pons‐Romero
迄今为止，还没有报道Fas系统参与原位肝移植（奥尔特）后复发性丙型肝炎病毒（HCV）感染。在25例因HCV相关肝硬化接受奥尔特的患者中，我们评估了肝细胞、肝硬化肝细胞上Fas抗原（FasAg）的表达和血清可溶性Fas（sFas）水平。用聚合酶链反应检测HCV病毒血症水平和HCV基因型。血清sFas水平通过酶免疫测定法测定。通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记（TUNEL）技术对脱蜡肝脏样本进行DNA片段化测定。免疫过氧化物酶法检测Fas抗原表达。16例患者有复发性HCV疾病的证据。复发性肝炎患者中表达Fas Ag的肝细胞数量和凋亡肝细胞百分比高于未复发者（分别为P <0.01和P <0.0001）。肝脏Fas Ag表达、小叶炎症强度（P = 0.007）和TUNEL指数（P <0.001）之间存在相关性。复发组sFas水平高于无复发组（P <0.04）。我们的结论是：（1）肝奥尔特后复发的HCV患者Fas表达上调，并与肝病分级相关;同样，复发的HCV肝炎患者sFas水平更高;以及（2）肝细胞表达Fas抗原和TUNEL法标记细胞核表明Fas系统介导的肝细胞凋亡可能在复发性HCV的发病机制中起作用奥尔特后肝炎
To date, there have been no reports of the involvement of the Fas system in recurrent hepatitis C virus (HCV) infection after orthotopic liver transplantation (OLT). In 25 patients who underwent OLT for HCV‐related liver cirrhosis, we evaluated the expression of the Fas antigen (FasAg) on hepatocytes, apoptic hepatocytes, and serum levels of soluble Fas (sFas). The level of HCV viremia and HCV genotype were determined by polymerase chain reaction. Serum sFas levels were determined by an enzyme immunoassay procedure. DNA fragmentation was determined by the terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end‐labeling (TUNEL) technique on deparaffinized liver samples. FasAg expression was evaluated by an immunoperoxidase method. Sixteen patients had evidence of recurrent HCV disease. The number of hepatocytes expressing FasAg and the percentage of apoptotic hepatocytes was greater among patients who developed recurrent hepatitis than among those who did not (P < .01 and P < .0001, respectively). There was a correlation between hepatic expression of FasAg, intensity of lobular inflammation (P = .007), and TUNEL index (P < .001). The levels of sFas were greater among the patients with recurrent HCV hepatitis than those without recurrent hepatitis (P < .04). We conclude that (1) Fas expression is up‐regulated in recurrent HCV after OLT and is related to the grading of liver disease; likewise, levels of sFas were greater in the patients with recurrent HCV hepatitis; and (2) the demonstration of hepatocytes with FasAg expression and the labeling of the nuclei by TUNEL assay suggest that hepatic apoptosis mediated by the Fas system may have a role in the pathogenesis of recurrent HCV hepatitis after OLT.
人类肝细胞产生一种抑制细胞凋亡的 FAS 亚型。
影响因子：
6.2
作者：
Krams,SM;Fox,CK;Beatty,PR;Cao,S;Villanueva,JC;Esquivel,CO;Martinez,OM
通讯作者：
Martinez,OM
影响因子：
3.7
作者：
Ray, RB;Meyer, K;Ray, R
通讯作者：
Ray, R
原位肝移植后复发性丙型肝炎的生化和组织学评估。
影响因子：
6.2
作者：
Shiffman,ML;Contos,MJ;Luketic,VA;Sanyal,AJ;Purdum3rd,PP;Mills,AS;Fisher,RA;Posner,MP
通讯作者：
Posner,MP