Expression and complex assembly of calcium‐binding proteins MRP8 and MRP14 during differentiation of murine myelomonocytic cells
Expression and complex assembly of calcium‐binding proteins MRP8 and MRP14 during differentiation of murine myelomonocytic cells
复制标题
小鼠骨髓单核细胞分化过程中钙结合蛋白 MRP8 和 MRP14 的表达和复合组装
作者：
M. Goebeler;J. Roth;U. Henseleit;C. Sunderkotter;C. Sorg
在本研究中，我们分析了最近克隆的两种钙结合蛋白MRP 8和MRP 14（均为S-100家族成员）在小鼠骨髓单核细胞中的表达和生化特性。当与转化的巨噬细胞系（M1、RMB.TG、WEHI.TG、J774 A、P388 D）和驻留的和渗出的腹膜和组织巨噬细胞中的其他标志物（Mac-1、F4/80）的表达相比时，发现MRP的表达限于粒细胞和巨噬细胞分化的不同阶段。同样，在L细胞条件培养基中培养期间，小鼠骨髓细胞中MRP 8/MRP 14的mRNA和蛋白水平降低。应用交联技术，证明了25、35和48 kd的非共价缔合的异聚体MRP 8/MRP 14复合物的形成。我们的数据表明，MRP 8/MRP 14的表达和复合是小鼠粒细胞和巨噬细胞分化的不同阶段的特征。对巨噬细胞的MRP 8/MRP 14+表型的分析可以进一步了解巨噬细胞分化的机制。
In the present study we analyzed expression and biochemical properties of the two recently cloned calcium‐binding proteins MRP8 and MRP14, both members of the S‐100 family, in murine myelomonocytic cells. Expression of MRPs was found to be restricted to granulocytes and distinct stages of macrophage differentiation when compared to the expression of other markers (Mac‐1, F4/80) in transformed macrophage cell lines (Ml, RMB.TG, WEHI.TG, J774A, P388D) and resident and exudate peritoneal and tissue macrophages. Similarly, mRNA and protein levels of MRP8/MRP14 in murine bone marrow cells decreased during culture in L cell‐conditioned medium. Applying a cross‐linking technique, the formation of noncovalently associated heteromeric MRP8/MRP14 complexes of 25, 35, and 48 kd was demonstrated. Our data suggest that MRP8/MRP14 expression and complexation are characteristic for granulocytes and distinct stages of macrophage differentiation in mice. Analysis of the MRP8/MRP14+ phenotype of macrophages may provide further insights into the mechanisms underlying macrophage differentiation.
小鼠高增殖能力造血祖细胞表达 Thy-1 抗原。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Boswell,HS;WadeJr,PM;Quesenberry,PJ
通讯作者：
Quesenberry,PJ
1 α,25-(OH)2 维生素 D3 增强编码 Ca(2) 结合蛋白 MRP-8 和 MRP-14 的基因表达。
DOI：
10.1152/ajpcell.1992.262.1.c235
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
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作者：
Sellmayer,A;Krane,SM;Ouellette,AJ;Bonventre,JV
通讯作者：
Bonventre,JV
巨噬细胞分化抗原在小鼠细胞系上的体外表达和诱导。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ralph,P;Ho,MK;Litcofsky,PB;Springer,TA
通讯作者：
Springer,TA