Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models.
Intracoronary administration of cardiac stem cells in mice: a new, improved technique for cell therapy in murine models.
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DOI：
10.1007/s00395-011-0180-1
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发表时间：
2011-09
影响因子：
9.5
通讯作者：
Bolli R
中科院分区：
文献类型：
--
作者：
Li Q;Guo Y;Ou Q;Chen N;Wu WJ;Yuan F;O'Brien E;Wang T;Luo L;Hunt GN;Zhu X;Bolli R
冠状动脉内干细胞输送模型，使转基因/基因靶向将是一个强大的工具，但仍然缺乏。为了解决这一差距，我们在再灌注心肌梗死（MI）小鼠模型中比较了lin−/c-kit+/GFP+心脏干细胞（CSC）的冠状动脉内和心肌内递送。Lin−/c-kit+/GFP+ CSCs成功地从GFP转基因心脏扩增，并培养了多达10代，没有可检测到的表型变化。MI后2天冠状动脉内输送CSC可显著缓解不良LV重塑和功能障碍，即使不上级，也至少与心肌内输送相当。与心肌内注射相比，冠状动脉内输注与梗死区域中CSC的更均匀分布以及该区域活组织的更大增加相关，表明新心肌细胞的形成更多。冠状动脉内CSC输送可改善梗死区域的功能，以及改善整体LV收缩和舒张功能，并降低LV扩张和LV扩张指数;这些效应的程度与心肌内注射后观察到的相似。我们的结论是，在再灌注心肌梗死的小鼠模型中，冠状动脉内CSC输注在限制LV重构和改善局部和全局LV功能方面至少与心肌内注射一样有效。冠状动脉内途径在细胞分布均匀性、心肌细胞再生和危险区域活组织数量方面似乎具有上级优势。据我们所知，这是第一个研究报告，在小鼠冠状动脉内输注干细胞是可行的和有效的。
A model of intracoronary stem cell delivery that enables transgenesis/gene targeting would be a powerful tool but is still lacking. To address this gap, we compared intracoronary and intramyocardial delivery of lin−/c-kit+/GFP+ cardiac stem cells (CSCs) in a murine model of reperfused myocardial infarction (MI). Lin−/c-kit+/GFP+ CSCs were successfully expanded from GFP transgenic hearts and cultured with no detectable phenotypic change for up to ten passages. Intracoronary delivery of CSCs 2 days post-MI resulted in significant alleviation of adverse LV remodeling and dysfunction, which was at least equivalent, if not superior, to that achieved with intramyocardial delivery. Compared with intramyocardial injection, intracoronary infusion was associated with a more homogeneous distribution of CSCs in the infarcted region and a greater increase in viable tissue in this region, suggesting greater formation of new cardiomyocytes. Intracoronary CSC delivery resulted in improved function in the infarcted region, as well as in improved global LV systolic and diastolic function, and in decreased LV dilation and LV expansion index; the magnitude of these effects was similar to that observed after intramyocardial injection. We conclude that, in the murine model of reperfused MI, intracoronary CSC infusion is at least as effective as intramyocardial injection in limiting LV remodeling and improving both regional and global LV function. The intracoronary route appears to be superior in terms of uniformity of cell distribution, myocyte regeneration, and amount of viable tissue in the risk region. To our knowledge, this is the first study to report that intracoronary infusion of stem cells in mice is feasible and effective.
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DOI：
10.1152/ajpheart.2000.279.4.h1698
发表时间：
2000-10-01
影响因子：
4.8
作者：
Feldman, MD;Erikson, JM;Freeman, GL
通讯作者：
Freeman, GL
影响因子：
20.1
作者：
Li, QH;Guo, Y;Bolli, R
通讯作者：
Bolli, R
影响因子：
3
作者：
Jakobsen, Jannik E.;Li, Juan;Mikkelsen, Jacob Giehm
通讯作者：
Mikkelsen, Jacob Giehm
影响因子：
20.1
作者：
Black, RG;Guo, YR;Auchampach, JA
通讯作者：
Auchampach, JA
影响因子：
37.8
作者：
Li Q;Guo Y;Ou Q;Cui C;Wu WJ;Tan W;Zhu X;Lanceta LB;Sanganalmath SK;Dawn B;Shinmura K;Rokosh GD;Wang S;Bolli R
通讯作者：
Bolli R