NGF and NT-3 exert differential effects on the expression of neuropeptides in the suprachiasmatic nucleus of rats withdrawn from ethanol treatment
NGF and NT-3 exert differential effects on the expression of neuropeptides in the suprachiasmatic nucleus of rats withdrawn from ethanol treatment
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NGF和NT-3对退出乙醇治疗的大鼠视交叉上核神经肽的表达产生不同的影响
DOI：
10.1016/s0006-8993(03)03030-0
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发表时间：
2003
期刊：
影响因子：
2.9
通讯作者：
M. Madeira
中科院分区：
文献类型：
--
作者：
M. Paula;P. Pereira;A. Cadete;M. Madeira
一些神经营养因子具有增强大脑多个区域神经肽表达的能力。最近的研究还表明，NGF灌流1个月以上，可以抵消慢性乙醇处理和戒断大鼠视交叉上核(SCN)中加压素(VP)和血管活性肠肽(VIP)合成和表达的减少。在目前的研究中，我们考察了中性营养素-3(NT-3)促进这种作用的有效性，因为SCN神经元表达这种神经营养素的高亲和力和低亲和力受体。连续10天脑室注射NT-3给长期酒精戒断大鼠。将VP和VIP免疫反应神经元的总数和平均体细胞体积与同期接受NGF或脑脊液治疗的对照组和戒断大鼠的估计值进行比较。脑脊液和NT-3的注入不能阻止乙醇戒断所致的多肽产生神经元数量的减少。相反，NGF输注使它们的数量增加到对照水平，并导致神经元肥大。我们的结果表明，与NGF不同，NT-3不具有增强SCN神经肽表达的能力。由于SCN神经元表达低亲和力的p75NTR，而这两种神经营养因子同样激活SCN神经元，我们的结果进一步表明，NGF对SCN神经元的影响不是由受体介导的。综上所述，我们的数据表明，NGF对SCN神经元施加的营养效应可能是间接机制，而不是直接的中性营养素信号。
Some neurotrophins have the capability of enhancing neuropeptide expression in several regions of the brain. It was also recently shown that NGF, infused over 1 month, offsets the decreased synthesis and expression of vasopressin (VP) and vasoactive intestinal polypeptide (VIP) in the suprachiasmatic nucleus (SCN) of rats submitted to chronic ethanol treatment and withdrawal. In the present study we examined the effectiveness of neutrotrophin-3 (NT-3) in promoting such effects, given that SCN neurons express both the high and the low affinity receptors for this neurotrophin. NT-3 was intraventricularly infused during 10 days to rats withdrawn from prolonged ethanol treatment. The total number, and the mean somatic volume, of VP- and VIP-immunoreactive neurons was compared with the estimates obtained from control rats and withdrawn rats treated with either NGF or cerebrospinal fluid during the same period. The infusion of cerebrospinal fluid and of NT-3 did not prevent the reduction in the number of peptide-producing neurons induced by withdrawal from ethanol treatment. Conversely, NGF infusion increased their number to control levels and led to neuronal hypertrophy. Our results show that, unlike NGF, NT-3 does not display the capacity of enhancing neuropeptide expression in the SCN. Because SCN neurons express the low affinity p75NTR, which is equally activated by both neurotrophins, our results additionally indicate that the effects of NGF upon SCN neurons are not receptor-mediated. Taken together, our data suggest that indirect mechanisms, rather than direct neutrophin signaling, are likely to mediate the trophic effects exerted by NGF upon SCN neurons.
影响因子：
4.1
作者：
Les Dees;C. Skelley
通讯作者：
Les Dees;C. Skelley
慢性乙醇对隔海马系统的影响：神经营养因子的作用？
DOI：
10.1111/j.1530-0277.1993.tb00719.x
发表时间：
1993
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Walker,DW;Heaton,MB;Lee,N;King,MA;Hunter,BE
通讯作者：
Hunter,BE
编码神经生长因子及其受体的基因在发育中的雌性大鼠下丘脑中表达。
DOI：
10.1016/0169-328x(91)90129-l
发表时间：
1991
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Ojeda,SR;Hill,DF;Katz,KH
通讯作者：
Katz,KH