A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life.
A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life.
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DOI：
10.1186/1471-2164-10-470
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发表时间：
2009-10-12
期刊：
影响因子：
4.4
通讯作者：
de Crécy-Lagard V
中科院分区：
文献类型：
--
作者：
Haas CE;Rodionov DA;Kropat J;Malasarn D;Merchant SS;de Crécy-Lagard V
COG0523蛋白与UreG家族的镍伴侣蛋白一样，是G3E家族gtp酶的一部分，与金属中心生物合成有关。尽管第一个编码cog0523的基因cobW在近20年前被发现，但人们对这个普遍存在的家族的其他成员的功能知之甚少。基于比较基因组学、文献和系统发育分析以及实验验证的结合，COG0523家族可以被划分为至少15个亚群。参与钴胺素合成的cow亚群仅代表该家族的一个小亚部分。另一个更大的亚群，根据在许多细菌基因组中假设的Zur结合位点下游存在相应的cog0523编码基因，被认为在锌限制反应中起主导作用。这些基因组中的Zur结合位点也与锌依赖酶的候选不依赖锌的类似物相关。最后，COG0523在锌稳态中的潜在作用并不局限于细菌。我们预测了COG0523与锌在古细菌中的调控之间的联系，并表明两个COG0523基因在真核参考生物莱茵衣藻（Chlamydomonas reinhardtii）缺锌时被诱导。本工作为通过实验方法探究COG0523成员在金属走私中的具体作用奠定了基础。基于系统发育和比较基因组学，金属特异性和蛋白质靶点可能在COG0523亚群之间有所不同。此外，COG0523的zr依赖性表达和锌依赖性蛋白的推测相似物可能代表了锌营养不足时锌的等级分布和锌节约的机制。
COG0523 proteins are, like the nickel chaperones of the UreG family, part of the G3E family of GTPases linking them to metallocenter biosynthesis. Even though the first COG0523-encoding gene, cobW, was identified almost 20 years ago, little is known concerning the function of other members belonging to this ubiquitous family. Based on a combination of comparative genomics, literature and phylogenetic analyses and experimental validations, the COG0523 family can be separated into at least fifteen subgroups. The CobW subgroup involved in cobalamin synthesis represents only one small sub-fraction of the family. Another, larger subgroup, is suggested to play a predominant role in the response to zinc limitation based on the presence of the corresponding COG0523-encoding genes downstream from putative Zur binding sites in many bacterial genomes. Zur binding sites in these genomes are also associated with candidate zinc-independent paralogs of zinc-dependent enzymes. Finally, the potential role of COG0523 in zinc homeostasis is not limited to Bacteria. We have predicted a link between COG0523 and regulation by zinc in Archaea and show that two COG0523 genes are induced upon zinc depletion in a eukaryotic reference organism, Chlamydomonas reinhardtii. This work lays the foundation for the pursuit by experimental methods of the specific role of COG0523 members in metal trafficking. Based on phylogeny and comparative genomics, both the metal specificity and the protein target(s) might vary from one COG0523 subgroup to another. Additionally, Zur-dependent expression of COG0523 and putative paralogs of zinc-dependent proteins may represent a mechanism for hierarchal zinc distribution and zinc sparing in the face of inadequate zinc nutrition.
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影响因子：
9.9
作者：
de Berardinis, Veronique;Vallenet, David;Castelli, Vanina;Besnard, Marielle;Pinet, Agnes;Cruaud, Corinne;Samair, Sumitta;Lechaplais, Christophe;Gyapay, Gabor;Richez, Celine;Durot, Maxime;Kreimeyer, Annett;Le Fevre, Francois;Schaechter, Vincent;Pezo, Valerie;Doering, Volker;Scarpelli, Claude;Medigue, Claudine;Cohen, Georges N.;Marliere, Philippe;Salanoubat, Marcel;Weissenbach, Jean
通讯作者：
Weissenbach, Jean
影响因子：
2.9
作者：
Campos-Bermudez, Valeria A.;Leite, Ney Ribeiro;Vila, Alejandro J.
通讯作者：
Vila, Alejandro J.
DOI：
10.1073/pnas.92.6.2333
发表时间：
1995-03-14
影响因子：
11.1
作者：
FU, CL;OLSON, JW;MAIER, RJ
通讯作者：
MAIER, RJ
影响因子：
7.4
作者：
Allen, Michael D.;Kropat, Janette;Merchant, Sabeeha S.
通讯作者：
Merchant, Sabeeha S.
影响因子：
2.8
作者：
Brichta, DM;Azad, KN;O'Donovan, GA
通讯作者：
O'Donovan, GA