Assembly and Maintenance of Lipids at the Bacterial Outer Membrane.
Assembly and Maintenance of Lipids at the Bacterial Outer Membrane.
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DOI：
10.1021/acs.chemrev.0c00587
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发表时间：
2021-05-12
期刊：
影响因子：
62.1
通讯作者：
Ruiz N
中科院分区：
文献类型：
--
作者：
Lundstedt E;Kahne D;Ruiz N
革兰氏阴性菌的外膜对于它们在恶劣环境中的生存至关重要，并提供对许多抗生素的内在抗性。这层膜很特别，它是一个高度不对称的脂质双层。外膜的内小叶含有磷脂，而与脂多糖（LPS）连接的脂肪酰基链构成外小叶的疏水部分。这种脂质不对称性，特别是磷脂从外小叶中被排除，是对疏水分子形成几乎不可穿透的屏障的关键，否则疏水分子可以穿过磷脂双层。人们早就知道这些脂质不是在外膜中产生的。现在认为，保守的多亚基蛋白质机器在这些脂质在内膜合成完成后提取这些脂质并将其转运到外膜。一个长期存在的问题是细胞如何建立和维持这种不对称的脂质双层与细胞包膜的其他组件的组装协调。本文综述了已被确定参与外膜生物合成的跨膜脂质转运系统：通过LPT机的LPS转运，通过Mla途径和最近提出的几种转运蛋白的磷脂转运。
The outer membrane of Gram-negative bacteria is essential for their survival in harsh environments and provides intrinsic resistance to many antibiotics. This membrane is remarkable; it is a highly asymmetric lipid bilayer. The inner leaflet of the outer membrane contains phospholipids whereas the fatty acyl chains attached to lipopolysaccharide (LPS) comprise the hydrophobic portion of the outer leaflet. This lipid asymmetry, and in particular the exclusion of phospholipids from the outer leaflet, is key to creating an almost impenetrable barrier to hydrophobic molecules that can otherwise pass through phospholipid bilayers. It has long been known that these lipids are not made in the outer membrane. It is now believed that conserved multi-subunit protein machines extract these lipids after their synthesis is completed at the inner membrane and transport them to the outer membrane. A longstanding question is how the cell builds and maintains this asymmetric lipid bilayer in coordination with the assembly of the other components of the cell envelope. This review describes the trans-envelope lipid transport systems that have been identified to participate in outer membrane biogenesis: LPS transport via the Lpt machine, and phospholipid transport via the Mla pathway and several recently proposed transporters.
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影响因子：
6.8
作者：
Chen J
通讯作者：
Chen J
DOI：
10.1126/science.1227215
发表时间：
2012-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chng SS;Xue M;Garner RA;Kadokura H;Boyd D;Beckwith J;Kahne D
通讯作者：
Kahne D
影响因子：
3.1
作者：
Cian, Melina;Giordano, Nicole;Dalebroux, Zachary D.
通讯作者：
Dalebroux, Zachary D.
影响因子：
30.3
作者：
Dalebroux, Zachary D.;Edrozo, Mauna B.;Miller, Samuel I.
通讯作者：
Miller, Samuel I.
影响因子：
3.1
作者：
Albiger, B;Johansson, L;Jonsson, AB
通讯作者：
Jonsson, AB