Importance of the Sgs1 helicase activity in DNA repair of Saccharomyces cerevisiae
Importance of the Sgs1 helicase activity in DNA repair of Saccharomyces cerevisiae
复制标题
Sgs1 解旋酶活性在酿酒酵母 DNA 修复中的重要性
作者：
J. Saffi;Valquíria Reis Pereira;J. Henriques
摘要酿酒酵母Sgs1蛋白与裂殖酵母Rqh1、人类Bloom和Werner蛋白一起，是大肠杆菌 家族的解旋酶。SGS1突变导致酵母细胞过早衰老，包括染色体外rDNA环的积累。我们最近发现SGS1p与DNA修复Rad16p蛋白相互作用，并在UVC、4-NQO和H_2O_2损伤中与Rad16p上位。因此，我们测试了含有解旋酶和C-末端结构域突变的SGS1菌株。我们在这里证明了SGS1的解旋酶活性对于SGS1突变表型的大多数元素是重要的，包括对UVC、4-NQO、H_2O_2、MMS和羟基尿素的敏感性。
Abstract The Saccharomyces cerevisiae Sgs1 protein, together with Schizosaccharomyces pombe Rqh1 and the human Bloom and Werner proteins, is a DNA helicase of the Escherichia coli RecQ family. Mutation of SGS1 causes premature aging in yeast cells, including the accumulation of extrachromosomal rDNA circles. We have recently shown that Sgs1p interacts with the DNA repair Rad16p protein and is epistatic to Rad16p for UVC, 4-NQO and H2O2 lesions. Therefore we tested sgs1 strains containing mutations in the helicase and C-terminal domains. We demonstrate here that the helicase activity of the Sgs1 is important for most elements of the sgs1 mutation phenotype, including sensitivity to UVC, 4-NQO, H2O2, MMS and hydroxyurea.
影响因子：
3.3
作者：
Kohji Kusano;M. Berres;William R. Engels
通讯作者：
Kohji Kusano;M. Berres;William R. Engels
维尔纳综合症。
DOI：
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发表时间：
1995
期刊：
Dermatologic clinics.
影响因子：
--
作者：
Duvic,M;Lemak,NA
通讯作者：
Lemak,NA
影响因子：
56.9
作者：
Sinclair, DA;Mills, K;Guarente, L
通讯作者：
Guarente, L