Dynamic behavior of cell-free mitochondrial DNA in human saliva.
Dynamic behavior of cell-free mitochondrial DNA in human saliva.
复制标题
人唾液中无细胞线粒体DNA的动态行为。
DOI：
10.1016/j.psyneuen.2022.105852
复制
发表时间：
2022-09
影响因子：
3.7
通讯作者：
Picard M
中科院分区：
文献类型：
--
作者：
Trumpff C;Rausser S;Haahr R;Karan KR;Gouspillou G;Puterman E;Kirschbaum C;Picard M
线粒体含有它们自己的基因组，可以在多种生物流体如血液和脑脊液中释放，作为无细胞线粒体DNA（cf-mtDNA）。在临床研究中，血液cf-mtDNA预测死亡率，较高的cf-mtDNA水平与精神和身体压力有关。然而，cf-mtDNA的动力学尚未确定，它是否可以像唾液中的其他神经内分泌标志物一样进行非侵入性测量尚未研究。在这里，我们报告了人类唾液中的cf-mtDNA，并在多周内建立了其自然的体内动态行为。在一个小的健康成年人的原则验证队列中，我们首先开发了一种方法来快速定量唾液cf-mtDNA而无需DNA分离，并证明唾液cf-mtDNA的存在。然后，我们采用这种方法对两名健康男性进行了密集的重复测量分析，在连续53-60天的4个每日时间点进行研究（每组n = 212-220次观察），同时测量类固醇激素，自我报告的每日情绪和健康相关行为。唾液cf-mtDNA表现出强烈的觉醒反应，在觉醒后30-45分钟达到两个数量级，每天变化，并与皮质醇觉醒反应中度相关。在探索性分析中，没有发现与自我报告的日常情绪/健康相关行为的一致关联，尽管这需要在未来的研究中进一步检查。cf-mtDNA的动态变化与唾液白细胞介素6（IL-6）呈负相关，与唾液cf-mtDNA的促炎作用不一致。唾液cf-mtDNA的高度动态行为为非侵入性研究打开了大门，研究mtDNA信号传导与人类健康的相关性。
Mitochondria contain their own genome that can be released in multiple biofluids such as blood and cerebrospinal fluid, as cell-free mitochondrial DNA (cf-mtDNA). In clinical studies, blood cf-mtDNA predicts mortality and higher cf-mtDNA levels are associated with mental and physical stress. However, the dynamics of cf-mtDNA has not been defined, and whether it can be measured non-invasively like other neuroendocrine markers in saliva has not been examined. Here we report cf-mtDNA in human saliva and establish its natural within-person dynamic behavior across multiple weeks. In a small proof-of-principle cohort of healthy adults, we first develop an approach to rapidly quantify salivary cf-mtDNA without DNA isolation, and demonstrate the existence of salivary cf-mtDNA. We then deploy this approach to perform an intensive repeated-measures analysis of two healthy men studied at 4 daily timepoints over 53–60 consecutive days (n = 212–220 observations each) with parallel measures of steroid hormones, self-reported daily mood, and health-related behaviors. Salivary cf-mtDNA exhibited a robust awakening response reaching up to two orders of magnitude 30–45 min after awakening, varied from day-to-day, and moderately correlated with the cortisol awakening response. In exploratory analyses, no consistent association with self-reported daily mood/health-related behaviors were found, although this requires further examination in future studies. Dynamic variation in cf-mtDNA was inversely related with salivary interleukin 6 (IL-6), inconsistent with a pro-inflammatory effect of salivary cf-mtDNA. The highly dynamic behavior of salivary cf-mtDNA opens the door to non-invasive studies examining the relevance of mtDNA signaling in relation to human health.
登录
查看更多内容
影响因子：
6.8
作者：
Hummel EM;Hessas E;Müller S;Beiter T;Fisch M;Eibl A;Wolf OT;Giebel B;Platen P;Kumsta R;Moser DA
通讯作者：
Moser DA
DOI：
10.1002/stem.2856
发表时间：
2018-09
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Hayakawa K;Chan SJ;Mandeville ET;Park JH;Bruzzese M;Montaner J;Arai K;Rosell A;Lo EH
通讯作者：
Lo EH
影响因子：
5.3
作者：
Bronkhorst AJ;Ungerer V;Diehl F;Anker P;Dor Y;Fleischhacker M;Gahan PB;Hui L;Holdenrieder S;Thierry AR
通讯作者：
Thierry AR
影响因子：
3.7
作者：
Caplin, A.;Chen, F. S.;Puterman, E.
通讯作者：
Puterman, E.
影响因子：
3.8
作者：
Almeida, DM;Wethington, E;Kessler, RC
通讯作者：
Kessler, RC