Expression of distinct RNAs from 3' untranslated regions.
Expression of distinct RNAs from 3' untranslated regions.
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DOI：
10.1093/nar/gkq1158
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发表时间：
2011-03
影响因子：
14.9
通讯作者：
Mattick JS
中科院分区：
文献类型：
--
作者：
Mercer TR;Wilhelm D;Dinger ME;Soldà G;Korbie DJ;Glazov EA;Truong V;Schwenke M;Simons C;Matthaei KI;Saint R;Koopman P;Mattick JS
真核生物基因的3′非翻译区（3′ UTR）调控mRNA的稳定性、定位和翻译。在这里，我们提出的证据表明，大量的3′ UTR在人类，小鼠和苍蝇也表达独立于相关的蛋白质编码序列，它们通常连接，可能是通过转录后切割。CAGE（capped analysis of gene expression）、SAGE（serial analysis of gene expression）和cDNA文库分析以及基因芯片表达谱分析表明，3′ UTR的独立表达是一种受调控的、保守的全基因组现象。我们详细描述了几种3′ UTR衍生RNA（uaRNA）在小鼠胚胎中的表达，通过原位杂交显示这些转录本以细胞和亚细胞特异性方式表达。我们的研究结果表明，3′UTR序列不仅可以顺式调节蛋白质表达，而且可以内在地和独立地反式调节蛋白质表达，可能是非编码RNA，这一结论得到了许多先前遗传学研究的支持。我们的研究结果提示了3′ UTR的新功能，以及在使用3′UTR序列探针分析基因表达时的谨慎。
The 3′ untranslated regions (3′UTRs) of eukaryotic genes regulate mRNA stability, localization and translation. Here, we present evidence that large numbers of 3′UTRs in human, mouse and fly are also expressed separately from the associated protein-coding sequences to which they are normally linked, likely by post-transcriptional cleavage. Analysis of CAGE (capped analysis of gene expression), SAGE (serial analysis of gene expression) and cDNA libraries, as well as microarray expression profiles, demonstrate that the independent expression of 3′UTRs is a regulated and conserved genome-wide phenomenon. We characterize the expression of several 3′UTR-derived RNAs (uaRNAs) in detail in mouse embryos, showing by in situ hybridization that these transcripts are expressed in a cell- and subcellular-specific manner. Our results suggest that 3′UTR sequences can function not only in cis to regulate protein expression, but also intrinsically and independently in trans, likely as noncoding RNAs, a conclusion supported by a number of previous genetic studies. Our findings suggest novel functions for 3′UTRs, as well as caution in the use of 3′UTR sequence probes to analyze gene expression.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
4.3
作者：
Dinger ME;Pang KC;Mercer TR;Mattick JS
通讯作者：
Mattick JS
影响因子：
4.6
作者：
Jenny, Andreas;Hachet, Olivier;Ephrussi, Anne
通讯作者：
Ephrussi, Anne
影响因子：
10.7
作者：
Hong, Xin;Scofield, Douglas G.;Lynch, Michael
通讯作者：
Lynch, Michael
哺乳动物转录组中的多种核酸内切切割位点取决于 microRNA、Drosha 和其他核酸酶。
影响因子：
16
作者：
Karginov, Fedor V.;Cheloufi, Sihem;Chong, Mark M. W.;Stark, Alexander;Smith, Andrew D.;Hannon, Gregory J.
通讯作者：
Hannon, Gregory J.