Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity.
Arabidopsis TNL-WRKY domain receptor RRS1 contributes to temperature-conditioned RPS4 auto-immunity.
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DOI：
10.3389/fpls.2013.00403
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发表时间：
2013
影响因子：
5.6
通讯作者：
Parker JE
中科院分区：
文献类型：
--
作者：
Heidrich K;Tsuda K;Blanvillain-Baufumé S;Wirthmueller L;Bautor J;Parker JE
在植物效应子触发免疫（ETI）中，细胞内核苷酸结合-富含亮氨酸重复序列（NLR）受体被特异性病原体效应子激活。拟南芥TIR（Toll-白细胞介素-1受体结构域）-NLR（表示为TNL）基因对RPS 4和RRS 1赋予对表达III型分泌效应子AvrRps 4的假单胞菌番茄致病变种（Pst）菌株DC 3000的抗性。AvrRps 4、RPS 4和TNL抗性调节剂EDS 1的核积累对于ETI是必需的。RRS 1具有C-末端“WRKY”转录因子DNA结合结构域，表明重要的RPS 4/RRS 1识别和/或抗性信号传导事件发生在核染色质上。在拟南芥登录Ws-0中，RPS 4 Ws/RRS 1 Ws等位基因对控制对Pst/AvrRps 4的抗性，伴随着宿主程序性细胞死亡（pcd）。在登录号Col-0中，RPS 4Col/RRS 1Col有效地限制了没有pcd的Pst/AvrRps 4生长。HA-StrepII标记的RPS 4Col（在35 S：RPS 4-HS系中）的组成型表达赋予温度调节的EDS 1依赖性自身免疫。在这里，我们表明，35 S：RPS 4-HS植物的高（28°C，非允许的）至中等（19°C，允许的）温度转变可用于在没有病原体效应物触发的情况下跟踪防御相关的转录动力学。通过比较35 S：RPS 4-HS与35 S：RPS 4-HS rrs 1 -11和35 S：RPS 4-HS eds 1 -2突变体的应答，我们确定RPS 4 Col自身免疫完全依赖于EDS 1，部分依赖于RRS 1Col。温度变化后24小时的基因表达微阵列数据的检查揭示了RRS 1Col对RPS 4Col重编程的EDS 1依赖性基因的一小部分的上调或下调的主要定量贡献。我们发现这些基因的启动子内WRKY转录因子结合W盒顺式元件的显着过度表达。我们的数据表明，RRS 1Col有助于温度调节的RPS 4Col自身免疫，并且与激活的RPS 4Col接合RRS 1Col用于抗性信号传导一致。
In plant effector-triggered immunity (ETI), intracellular nucleotide binding-leucine rich repeat (NLR) receptors are activated by specific pathogen effectors. The Arabidopsis TIR (Toll-Interleukin-1 receptor domain)-NLR (denoted TNL) gene pair, RPS4 and RRS1, confers resistance to Pseudomonas syringae pv tomato (Pst) strain DC3000 expressing the Type III-secreted effector, AvrRps4. Nuclear accumulation of AvrRps4, RPS4, and the TNL resistance regulator EDS1 is necessary for ETI. RRS1 possesses a C-terminal “WRKY” transcription factor DNA binding domain suggesting that important RPS4/RRS1 recognition and/or resistance signaling events occur at the nuclear chromatin. In Arabidopsis accession Ws-0, the RPS4Ws/RRS1Ws allelic pair governs resistance to Pst/AvrRps4 accompanied by host programed cell death (pcd). In accession Col-0, RPS4Col/RRS1Col effectively limits Pst/AvrRps4 growth without pcd. Constitutive expression of HA-StrepII tagged RPS4Col (in a 35S:RPS4-HS line) confers temperature-conditioned EDS1-dependent auto-immunity. Here we show that a high (28°C, non-permissive) to moderate (19°C, permissive) temperature shift of 35S:RPS4-HS plants can be used to follow defense-related transcriptional dynamics without a pathogen effector trigger. By comparing responses of 35S:RPS4-HS with 35S:RPS4-HS rrs1-11 and 35S:RPS4-HS eds1-2 mutants, we establish that RPS4Col auto-immunity depends entirely on EDS1 and partially on RRS1Col. Examination of gene expression microarray data over 24 h after temperature shift reveals a mainly quantitative RRS1Col contribution to up- or down-regulation of a small subset of RPS4Col-reprogramed, EDS1-dependent genes. We find significant over-representation of WRKY transcription factor binding W-box cis-elements within the promoters of these genes. Our data show that RRS1Col contributes to temperature-conditioned RPS4Col auto-immunity and are consistent with activated RPS4Col engaging RRS1Col for resistance signaling.
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影响因子：
6.7
作者：
García AV;Blanvillain-Baufumé S;Huibers RP;Wiermer M;Li G;Gobbato E;Rietz S;Parker JE
通讯作者：
Parker JE
WRKY8 转录因子通过介导拟南芥中的脱落酸和乙烯信号传导在 TMV-cg 防御反应中发挥作用
DOI：
10.1073/pnas.1221347110
发表时间：
2013-05-21
影响因子：
11.1
作者：
Chen, Ligang;Zhang, Liping;Yu, Diqiu
通讯作者：
Yu, Diqiu
影响因子：
11.4
作者：
Feys, BJ;Moisan, LJ;Parker, JE
通讯作者：
Parker, JE
DOI：
10.1073/pnas.1230660100
发表时间：
2003-06-24
影响因子：
11.1
作者：
Deslandes, L;Olivier, J;Marco, Y
通讯作者：
Marco, Y
影响因子：
6.7
作者：
Kim SH;Gao F;Bhattacharjee S;Adiasor JA;Nam JC;Gassmann W
通讯作者：
Gassmann W