Microscopy of membrane lipids: how precisely can we define their distribution?
Microscopy of membrane lipids: how precisely can we define their distribution?
复制标题
膜脂显微镜：我们如何精确地定义它们的分布？
DOI：
10.1042/bse0570081
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Takatori S and Fujimoto T
中科院分区：
文献类型：
--
作者：
Takatori S;Tatematsu T;Cheng J;Matsumoto J;Akano T;Fujimoto T;Takatori S and Fujimoto T
膜脂质通过形成脂质双层而形成生物膜的基本框架，但越来越清楚的是，各个脂质种类发挥着不同的功能作用。然而，与蛋白质相比，人们对脂质如何在膜中分布知之甚少。有几种微观方法可用于研究活细胞中的膜脂质动力学，但在亚微米尺度上定义脂质的分布很困难，因为脂质在膜中快速扩散，并且大多数脂质不与常用作固定剂的醛发生反应。快速冷冻似乎是瞬时停止脂质运动并捕获感兴趣时刻的分子定位的唯一实用方法。使用冷冻切片、树脂切片和冷冻断裂复制品的电子显微镜方法可用于观察速冻样品中的脂质。采用冷冻断裂复制品的方法是独特的，因为它不需要化学处理并提供膜的二维视图。
Membrane lipids form the basic framework of biological membranes by forming the lipid bilayer, but it is becoming increasingly clear that individual lipid species play different functional roles. However, in comparison with proteins, relatively little is known about how lipids are distributed in the membrane. Several microscopic methods are available to study membrane lipid dynamics in living cells, but defining the distribution of lipids at the submicrometre scale is difficult, because lipids diffuse quickly in the membrane and most lipids do not react with aldehydes that are commonly used as fixatives. Quick-freezing appears to be the only practical method by which to stop the lipid movement instantaneously and capture the molecular localization at the moment of interest. Electron microscopic methods, using cryosections, resin sections, and freeze-fracture replicas are used to visualize lipids in quick-frozen samples. The method that employs the freeze-fracture replica is unique in that it requires no chemical treatment and provides a two-dimensional view of the membrane.
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4.5
作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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