The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins [published erratum appears in J Cell Biol 1991 Feb;112(3):following 513]
The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins [published erratum appears in J Cell Biol 1991 Feb;112(3):following 513]
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透明质酸受体是细胞表面糖蛋白 CD44 (H-CAM) 家族的成员[已发表的勘误表出现在 J Cell Biol 1991 Feb；112(3):following 513]
作者：
Martine Culty;K. Miyake;P. Kincade;E. Sikorski;Eugene C. Butcher;Charles B. Underhill;E. Silorski
本研究旨在确定透明质酸受体和CD44(H-CAM)之间的关系，CD44(H-CAM)是分子量相似的细胞表面糖蛋白，参与细胞黏附。在最初的实验中，一组针对CD44的单抗被测试了它们与透明质酸受体发生交叉反应的能力。这些抗体从小鼠和人类细胞的洗涤剂提取物中免疫沉淀[~3H]透明质酸结合活性，表明透明质酸受体与CD44相同。此外，其中一种抗体(抗鼠CD44的KM-201)直接阻断了标记的透明质酸与受体的结合，并抑制了SV-3T3细胞依赖于透明质酸的聚集。在淋巴细胞归巢过程中，CD44也参与了淋巴细胞与高内皮微静脉的结合。有趣的是，阻断淋巴细胞与粘膜淋巴组织高内皮微静脉结合的单抗Hermes-3对标记的透明质酸的结合没有影响。此外，阻断透明质酸结合的药物(透明质酸酶、过量透明质酸和特异性抗体)对淋巴细胞与淋巴结和粘膜淋巴组织的高内皮细胞的结合没有明显影响。因此，CD44似乎至少有两个不同的功能结构域，一个用于结合透明质酸，另一个参与与粘膜高内皮微静脉的相互作用。
The present study was undertaken to determine the relationship between the hyaluronate receptor and CD44 (H-CAM), cell-surface glycoproteins of similar molecular weights that have been implicated in cell adhesion. In initial experiments, a panel of monoclonal antibodies directed against CD44 were tested for their ability to cross react with the hyaluronate receptor. These antibodies immunoprecipitated [3H]hyaluronate binding activity from detergent extracts of both mouse and human cells, indicating that the hyaluronate receptor is identical to CD44. In addition, one of these antibodies (KM-201 to mouse CD44) directly blocked the binding of labeled hyaluronate to the receptor and inhibited hyaluronate dependent aggregation of SV-3T3 cells. CD44 has also been implicated in lymphocyte binding to high endothelial venules during lymphocyte homing. Interestingly, the monoclonal antibody Hermes- 3, which blocks lymphocyte binding to the high endothelial venules of mucosal lymphoid tissue, had no effect on the binding of labeled hyaluronate. Furthermore, the binding of lymphocytes to high endothelial cells of lymph nodes and mucosal lymphoid tissue was not significantly affected by treatment with agents that block the binding of hyaluronate (hyaluronidase, excess hyaluronate and specific antibodies). Thus, CD44 appears to have at least two distinct functional domains, one for binding hyaluronate and another involved in interactions with mucosal high endothelial venules.
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透明质酸受体的单克隆抗体可抑制巨噬细胞和成纤维细胞的聚集。
影响因子：
3.7
作者：
Green,SJ;Tarone,G;Underhill,CB
通讯作者：
Underhill,CB
亲本和病毒转化细胞系表面的透明质酸受体：结合和聚集研究。
影响因子：
3.7
作者：
Underhill,CB;Toole,BP
通讯作者：
Toole,BP
DOI：
--
发表时间：
1983-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Underhill;G. Chi‐Rosso;B. Toole
通讯作者：
C. Underhill;G. Chi‐Rosso;B. Toole
III 类胶原受体的表征，一种在有核人类细胞中表达的磷酸化跨膜糖蛋白。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Carter,WG;Wayner,EA
通讯作者：
Wayner,EA
DOI：
10.1002/9780470513774.ch6
发表时间：
2007-09
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
C. Underhill
通讯作者：
C. Underhill