A new plasmid carrying mphA causes prevalence of azithromycin resistance in enterotoxigenic Escherichia coli serogroup O6
A new plasmid carrying mphA causes prevalence of azithromycin resistance in enterotoxigenic Escherichia coli serogroup O6
复制标题
携带 mphA 的新质粒导致产肠毒素大肠杆菌 O6 血清群普遍存在阿奇霉素耐药性
DOI：
10.1186/s12866-020-01927-z
复制
发表时间：
2020-03
期刊：
影响因子：
4.2
通讯作者：
Sun Yansong
中科院分区：
文献类型：
--
作者：
Xiang Ying;Wu Feng;Chai Yinghui;Xu Xuebin;Yang Lang;Tian Sai;Zhang Haoran;Li Yinxia;Yang Chaojie;Liu Hongbo;Qiu Shaofu;Song Hongbin;Sun Yansong
Background At present, azithromycin has become an effective treatment for severe diarrhea caused by EnterotoxigenicEscherichia coli(ETEC) infection. However, enterobacteria have begun to develop resistance to azithromycin and have attracted attention in recent years. This study conducted to described the emergence of a high proportion of azithromycin-resistant ETEC serogroup O6 strains in Shanghai and to analyzed the mechanisms of azithromycin resistance. Results Strains from adult diarrhea patients with ETEC serogroup O6 infections were collected by Shanghai Diarrhea Surveillance Network and the Foodborne Surveillance Network from 2016 to 2018. We tested 30 isolates of ETEC O6 serogroup, 26 of which were resistant to azithromycin. Phylogenetic analysis revealed that these ETEC serogroup O6 strains have formed an independent dominant clone. S1-PFGE and southern blotting revealed the presence of themphAgene on the 103 kb plasmid. Illumina and Nanopore sequencing and plasmid coverage analysis further confirmed that azithromycin-resistant strains carried a novel IncFII plasmid harboringmphAandblaTEM-1 resistance genes. Conclusions This is the first study to report a high proportion of azithromycin resistance in a particular ETEC serogroup due to a specific plasmid carryingmphA. Our findings indicate the rapid spread of azithromycin resistance, highlighting the urgency of stringent surveillance and control measure.
登录
查看更多内容
影响因子：
5.3
作者：
通讯作者：
--
影响因子：
4.6
作者：
Gomes, Claudia;Ruiz-Roldan, Lidia;Ruiz, Joaquim
通讯作者：
Ruiz, Joaquim
影响因子：
3.7
作者：
Walker BJ;Abeel T;Shea T;Priest M;Abouelliel A;Sakthikumar S;Cuomo CA;Zeng Q;Wortman J;Young SK;Earl AM
通讯作者：
Earl AM
DOI：
10.1016/j.ijantimicag.2017.01.021
发表时间：
2017-06-01
影响因子：
10.8
作者：
Liang, Quanhui;Yin, Zhe;Zhou, Dongsheng
通讯作者：
Zhou, Dongsheng
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.