Pleural Fluid Has Pro-Growth Biological Properties Which Enable Cancer Cell Proliferation.
Pleural Fluid Has Pro-Growth Biological Properties Which Enable Cancer Cell Proliferation.
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DOI：
10.3389/fonc.2021.658395
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发表时间：
2021
影响因子：
4.7
通讯作者：
Psallidas I
中科院分区：
文献类型：
--
作者：
Asciak R;Kanellakis NI;Yao X;Abd Hamid M;Mercer RM;Hassan M;Bedawi EO;Dobson M;Fsadni P;Montefort S;Dong T;Rahman NM;Psallidas I
恶性胸膜间皮瘤（MPM）或胸膜转移瘤患者常出现恶性胸腔积液（MPE）。本研究旨在分析胸水对癌细胞的影响。将来自MPE（MPM、乳腺癌、肺腺癌）的已建立的患者来源的癌细胞培养物接种在100%胸腔液（渗出物MPM MPE、漏出物MPE、非MPE漏出液）中，并监测增殖。此外，通过将细胞接种于100% MPE液体中，尝试建立来自MPE标本的新MPM细胞培养物。所有已建立的癌细胞培养物在不同类型的胸腔积液中以相似的生长速率增殖。原代MPM细胞培养成功率与MPE液与全培养基相似。无论胸腔积液的来源如何，单独的胸腔积液足以在体外进行癌细胞增殖。这些结果支持胸膜液具有重要的促生长生物学特性的假设，但这种作用的机制尚不清楚，可能不是恶性积液特异性的。
Patients with malignant pleural mesothelioma (MPM) or pleural metastases often present with malignant pleural effusion (MPE). This study aimed to analyze the effect of pleural fluid on cancer cells. Established patient-derived cancer cell cultures derived from MPE (MPM, breast carcinoma, lung adenocarcinoma) were seeded in 100% pleural fluid (exudate MPM MPE, transudate MPE, non-MPE transudate fluid) and proliferation was monitored. In addition, the establishment of new MPM cell cultures, derived from MPE specimens, was attempted by seeding the cells in 100% MPE fluid. All established cancer cell cultures proliferated with similar growth rates in the different types of pleural fluid. Primary MPM cell culture success was similar with MPE fluid as with full culture medium. Pleural fluid alone is adequate for cancer cell proliferation in vitro, regardless of the source of pleural fluid. These results support the hypothesis that pleural fluid has important pro-growth biological properties, but the mechanisms for this effect are unclear and likely not malignant effusion specific.
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影响因子：
30.8
作者：
Lee, Jin-Ku;Liu, Zhaoqi;Nam, Do-Hyun
通讯作者：
Nam, Do-Hyun
DOI：
10.1158/1541-7786.mcr-15-0189
发表时间：
2016-01
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Goodspeed A;Heiser LM;Gray JW;Costello JC
通讯作者：
Costello JC
影响因子：
9.6
作者：
Chen, YM;Yang, WK;Perng, RP
通讯作者：
Perng, RP
影响因子：
3.4
作者：
Bibby, Anna C.;Dorn, Patrick;Cardillo, Giuseppe
通讯作者：
Cardillo, Giuseppe
影响因子：
11.2
作者：
Stathopoulos, Georgios T.;Kollintza, Androniki;Kalomenidis, Ioannis
通讯作者：
Kalomenidis, Ioannis