Sprouting and Regeneration of Lesioned Corticospinal Tract Fibres in the Adult Rat Spinal Cord
Sprouting and Regeneration of Lesioned Corticospinal Tract Fibres in the Adult Rat Spinal Cord
复制标题
成年大鼠脊髓损伤皮质脊髓束纤维的萌芽和再生
DOI：
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发表时间：
1993
影响因子：
3.4
通讯作者：
Martin E. Schwab
中科院分区：
文献类型：
--
作者：
L. Schnell;Martin E. Schwab
我们研究了组织移植物和针对髓鞘相关神经突生长抑制蛋白的抗体（IN-1）对大鼠皮质脊髓束（CST）发芽和再生的影响。将胚胎脊髓组织移植到幼年大鼠双侧下胸段脊髓横断损伤处，可明显增加损伤脊髓束的发芽。这种发芽效应可能是由移植物释放的可溶性因子引起的，并被IN-1抗体增强。通常观察到的存活时间延长的病变CST纤维的回缩也减少了移植物的存在。尽管移植物具有这些生长促进作用，但CST芽进入尾侧脊髓的再生延长取决于髓鞘相关抑制蛋白的中和。在对照组（无抗体或对照抗体）中，仅27%的动物显示CST纤维伸长超过0.7 mm的发芽距离。这些纤维生长至1.8 mm的最大长度（平均值±SEM，1.2±0.1）。相比之下，60%的IN-1处理的含移植物的大鼠显示出>0.7 mm的伸长，并且这些纤维生长至10.1 mm（4.6±0.5）。再生纤维通过剩余的组织桥穿过病变部位。无论是胚胎脊髓移植物还是各种植入的桥接材料都不能作为再生CST轴突的基质。
We have studied the effects of tissue transplants and antibodies (IN‐1) against the myelin‐associated neurite growth inhibitory proteins on sprouting and regeneration of the rat corticospinal tract (CST). Transplantation of embryonic spinal cord tissue into bilateral transection lesions of the lower thoracic spinal cord in young adult rats resulted in a marked increase of the sprouting of the lesioned CST. This sprouting effect was probably elicited by soluble factors released from the transplants, and was enhanced by the IN‐1 antibodies. The retraction of lesioned CST fibres normally observed with prolonged survival times was also reduced by the presence of transplants. In spite of these growth‐promoting effects of the transplants, the regenerative elongation of CST sprouts into the caudal spinal cord was dependent upon the neutralization of the myelin‐associated inhibitory proteins. In the controls (no antibodies or control antibodies) only 27% of the animals showed elongation of CST fibres exceeding the sprouting distance of 0.7 mm. These fibres grew to a maximal length of 1.8 mm (mean±SEM, 1.2±0.1). In contrast, 60% of the IN‐1‐treated, transplant‐containing rats showed elongations of >0.7 mm, and these fibres grew up to 10.1 mm (4.6±0.5). Regenerating fibres crossed the lesion site through remaining tissue bridges. Neither embryonic spinal cord transplants nor a variety of implanted bridge materials could serve as a substrate for the regenerating CST axons.
DOI：
10.1073/pnas.89.7.2634
发表时间：
1992-04-01
影响因子：
11.1
作者：
LAYWELL, ED;DORRIES, U;STEINDLER, DA
通讯作者：
STEINDLER, DA