Distinct Subfamilies of Odorant Binding Proteins in Locust (Orthoptera, Acrididae): Molecular Evolution, Structural Variation, and Sensilla-Specific Expression.
Distinct Subfamilies of Odorant Binding Proteins in Locust (Orthoptera, Acrididae): Molecular Evolution, Structural Variation, and Sensilla-Specific Expression.
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DOI：
10.3389/fphys.2017.00734
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发表时间：
2017
影响因子：
4
通讯作者：
Pregitzer P
中科院分区：
文献类型：
--
作者：
Jiang X;Krieger J;Breer H;Pregitzer P
气味结合蛋白（OBPs）在昆虫嗅觉中起着重要作用，促进气味分子在感受器淋巴中的运输。虽然大多数研究集中在鳞翅目和双翅目物种上，但我们对直翅目物种的了解仍然非常有限。本研究对荒漠蝗（Schistocerca gregaria）的OBPs进行了研究。我们鉴定出了14个来自S. gregaria触角转录组编码SgreOBPs的转录本，并总结了SgreOBPs与其他三种蝗虫的OBPs的系统发育关系。已经鉴定出两个保守的经典OBPs亚家族，命名为I-A和II-A，显示出共同和亚家族特异性氨基酸基序。I-A亚家族和II-A亚家族的obp具有明显的进化特征。研究了两个亚科OBP成员的表面拓扑结构和内部空腔结构。触角地形表达显示了来自I-A亚家族和II-A亚家族的SgreOBPs不同的感受器和细胞特异性表达模式。这些发现首次深入了解了蝗虫OBP的分子和进化特征，以及它们在触角中的表达，这可能是揭示不同OBP亚家族在蝗虫嗅觉中的具体作用的第一步。
Odorant binding proteins (OBPs) play an important role in insect olfaction, facilitating transportation of odorant molecules in the sensillum lymph. While most of the researches are concentrated on Lepidopteran and Dipteran species, our knowledge about Orthopteran species is still very limited. In this study, we have investigated OBPs of the desert locust Schistocerca gregaria, a representative Orthopteran species. We have identified 14 transcripts from a S. gregaria antennal transcriptome encoding SgreOBPs, and recapitulated the phylogenetic relationship of SgreOBPs together with OBPs from three other locust species. Two conserved subfamilies of classic OBPs have been identified, named I-A and II-A, exhibiting both common and subfamily-specific amino acid motifs. Distinct evolutionary features were observed for subfamily I-A and II-A OBPs. Surface topology and interior cavity were elucidated for OBP members from the two subfamilies. Antennal topographic expression revealed distinct sensilla- and cellular- specific expression patterns for SgreOBPs from subfamily I-A and II-A. These findings give first insight into the repertoire of locust OBPs with respect to their molecular and evolutionary features as well as their expression in the antenna, which may serve as an initial step to unravel specific roles of distinct OBP subfamilies in locust olfaction.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4.8
作者：
Lartigue, A;Gruez, A;Cambillau, C
通讯作者：
Cambillau, C
DOI：
10.1007/s11068-005-5054-8
发表时间：
2005-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Maida, R;Mameli, M;Steinbrecht, RA
通讯作者：
Steinbrecht, RA
影响因子：
7
作者：
Hekmat-Scafe, DS;Scafe, CR;Tanouye, MA
通讯作者：
Tanouye, MA
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS