The kinetics of embryo drying in Drosophila melanogaster as a function of the steps in permeabilization: Experimental
The kinetics of embryo drying in Drosophila melanogaster as a function of the steps in permeabilization: Experimental
复制标题
果蝇胚胎干燥动力学作为透化步骤的函数：实验
DOI：
10.1016/0022-1910(95)00122-0
复制
发表时间：
1996
影响因子：
2.2
通讯作者：
P. Mazur
中科院分区：
文献类型：
--
作者：
P. Schreuders;J. Kassis;K. W. Cole;U. Schneider;A. Mahowald;P. Mazur
果蝇胚胎被外层绒毛膜和内部卵黄膜包围，这是水运动的有效屏障。2.5%次氯酸盐可以去除绒毛膜(1)。如果用异丙醇(2)除去地表水，则含有0.3% 1-丁醇(3)的庚烷可使卵黄膜透水。在这里，我们定量地确定了这三个步骤对胚胎透水性的影响。通过将胚胎暴露在22-23°C和大约。56%的r / h，随着时间的推移确定体重减轻。我们还测定了三个渗透步骤对胚胎初始、与空气平衡后和完全干燥后的水和固体含量的影响。完整的胚胎含有76%的水和24%的固体。连续的渗透步骤使前者小幅增加，后者小幅减少。完整的胚胎需要5 - 12小时才能失去一半的水分，较长的时间适用于年龄较大的胚胎。经过(1)、(1)+(2)、(1)+(2)、(1)+(2)+(3)渗透步骤后，50%失水时间分别减少到1.6、1.9、0.1 h。
The Drosophila embryo is surrounded by an outer chorion and an inner vitelline membrane that are an effective barrier to the movement of water. The chorion can be removed by 2.5% hypochlorite (1). If surface water is then removed by isopropanol (2), the vitelline membrane can be rendered permeable to water by heptane containing 0.3% 1-butanol (3). Here, we determined quantitatively the effects of these three steps on the permeability of the embryo to water. Permeability was assessed by exposing embryos to air at 22–23 °C and approx. 56% r.h. and determining weight loss with time. We also determined the effect of the three permeabilization steps on the water and solids contents of the embryos initially, after equilibration with air, and after complete drying. The intact embryo contains 76% (w w ) water and 24% solids. The successive permeabilization steps produce small increases in the former and small decreases in the latter. Intact embryos require 5–12h to lose half their water, the longer times being applicable to older embryos. After permeabilization steps (1), (1) + (2), and (1) + (2) + (3) the times for 50% water loss decrease to 1.6, 1.9, and 0.1 h, respectively.
影响因子：
2.7
作者：
Myers,SP;Pitt,RE;Lynch,DV;Steponkus,PL
通讯作者：
Steponkus,PL
DOI：
10.1115/1.2895715
发表时间：
1994
期刊：
Journal of biomechanical engineering
影响因子：
--
作者：
Schreuders,PD;Diller,KR;BeamanJr,JJ;Paynter,HM
通讯作者：
Paynter,HM
影响因子：
2.7
作者：
Lynch,DV;Lin,TT;Myers,SP;Leibo,SP;Macintyre,RJ;Pitt,RE;Steponkus,PL
通讯作者：
Steponkus,PL