De novo lipogenesis is elicited dramatically in human hepatocellular carcinoma especially in hepatitis C virus-induced hepatocellular carcinoma.
De novo lipogenesis is elicited dramatically in human hepatocellular carcinoma especially in hepatitis C virus-induced hepatocellular carcinoma.
复制标题
在人类肝细胞癌中，特别是在丙型肝炎病毒诱导的肝细胞癌中，显着引发从头脂肪生成
作者：
Li S;Liu R;Pan Q;Wang G;Cheng D;Yang J;Chen H;Xu G
肝细胞癌（HCC）是全球癌症死亡的第三大原因。据报道，异常的新生脂肪生成与肝癌的发生有关。在目前的研究中，研究了乙型肝炎病毒（HBV）、丙型肝炎病毒（HCV）或非病毒因素诱导的人肝癌样本的新生脂肪生成及其与患者生存率的关系。采用real - time - PCR （RT - PCR）和western blot检测肝脏脂肪生成转录因子和脂质合成酶的mRNA和蛋白水平。使用癌症基因组图谱（TCGA）数据分析基因表达与患者生存的关系。与对照肝脏相比，人类HCC中脂肪生成途径调节因子如AKT2、SREBP1c、PPARγ和脂肪生成酶如ACC和FAS增加。值得注意的是，与HBV - HCC和非病毒性HCC相比，HCV - HCC中新生脂肪生成的增加更为明显。在HCV‐HCC中，FAS和ACC的高表达与较差的总生存期（OS）相关。在HCV‐HCC中，脂肪生成基因面板的高表达与不良OS显著相关，但在HBV‐HCC或非病毒性HCC中无显著相关性。总之，在人类HCC中，尤其是HCV‐HCC，可显著促进脂肪的新生形成。
Abstract Hepatocellular carcinoma (HCC) is the third leading cause of cancer deaths worldwide. Abnormal de novo lipogenesis is reported to be involved in hepatocarcinogenesis. In current study, de novo lipogenesis and its association with patient survival rate were investigated in human HCC samples induced by hepatitis B virus (HBV), hepatitis C virus (HCV), or nonviral factors. Hepatic mRNA and protein levels of lipogenic transcription factors and lipid synthesis enzymes were examined by realtime‐PCR (RT‐PCR) and western blot. Association of gene expression and patient survival was analyzed using The Cancer Genome Atlas (TCGA) data. Lipogenic pathway regulators such as AKT2, SREBP1c, PPARγ, and lipogenic enzymes such as ACC and FAS were increased in human HCC when compared with control livers. Notably, a more robust increase in de novo lipogenesis was observed in HCV‐HCC when compared to HBV‐HCC and nonviral HCC. High FAS and ACC expression correlated with poor overall survival (OS) in HCV‐HCC. High expression of lipogenesis gene panel significantly correlated with poor OS in HCV‐HCC, but not in HBV‐HCC or nonviral HCC. In sum, de novo lipogenesis is stimulated dramatically in human HCC especially in HCV‐HCC.
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DOI：
10.1073/pnas.202608199
发表时间：
2002-11-26
影响因子：
11.1
作者：
Su, AI;Pezacki, JP;Chisari, FV
通讯作者：
Chisari, FV
影响因子：
4.3
作者：
Chang, Ming-Ling
通讯作者：
Chang, Ming-Ling
影响因子：
10.9
作者：
Syed, Gulam H.;Amako, Yutaka;Siddiqui, Aleem
通讯作者：
Siddiqui, Aleem
影响因子：
29.4
作者：
El-Serag, HB;Tran, T;Everhart, JE
通讯作者：
Everhart, JE
影响因子：
158.5
作者：
Calle, EE;Rodriguez, C;Thun, MJ
通讯作者：
Thun, MJ