A novel pyrimidin-like plant activator stimulates plant disease resistance and promotes growth.
A novel pyrimidin-like plant activator stimulates plant disease resistance and promotes growth.
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DOI：
10.1371/journal.pone.0123227
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yao N
中科院分区：
文献类型：
--
作者：
Sun TJ;Lu Y;Narusaka M;Shi C;Yang YB;Wu JX;Zeng HY;Narusaka Y;Yao N
植物激活剂是诱导植物对广谱病原体的防御反应的化学物质。在此，我们鉴定了一种新的潜在植物激活剂，5-（环丙基甲基）-6-甲基-2-（2-吡啶基）嘧啶-4-醇，命名为PPA（嘧啶型植物激活剂）。与水杨酸（SA）类似物苯并噻二唑S-甲酯（BTH）相比，PPA完全溶于水，在低浓度下能增加水稻和拟南芥的鲜重。此外，PPA还促进了侧根的发育。基因芯片和实时荧光定量PCR结果显示，PPA处理的叶片未受到病原菌的攻击，表现出活性氧（ROS），防御和SA相关基因的上调。在细菌感染过程中，与BTH预处理的植物相比，PPA预处理的拟南芥植物表现出显着降低的疾病症状和更早和更强的ROS爆发。显微镜观察发现H2 O2在胞内细菌周围的胞质溶胶、质膜和细胞壁中积累，也在细菌细胞壁上积累，表明H2 O2直接参与了细菌的杀灭。ROS相关基因表达的增加也支持了这一观察结果。这些结果表明，PPA通过植物氧化还原系统增强植物对病原菌入侵的防御能力，并且作为一种水溶性化合物，可以促进植物生长，在农业上具有广泛的应用前景。
Plant activators are chemicals that induce plant defense responses to a broad spectrum of pathogens. Here, we identified a new potential plant activator, 5-(cyclopropylmethyl)-6-methyl-2-(2-pyridyl)pyrimidin-4-ol, named PPA (pyrimidin-type plant activator). Compared with benzothiadiazole S-methyl ester (BTH), a functional analog of salicylic acid (SA), PPA was fully soluble in water and increased fresh weight of rice (Oryza sativa) and Arabidopsis plants at low concentrations. In addition, PPA also promoted lateral root development. Microarray data and real-time PCR revealed that PPA-treated leaves not challenged with pathogen showed up-regulation of genes related to reactive oxygen species (ROS), defenses and SA. During bacterial infection, Arabidopsis plants pretreated with PPA showed dramatically decreased disease symptoms and an earlier and stronger ROS burst, compared with plants pretreated with BTH. Microscopy revealed that H2O2 accumulated in the cytosol, plasma membrane and cell wall around intracellular bacteria, and also on the bacterial cell wall, indicating that H2O2 was directly involved in killing bacteria. The increase in ROS-related gene expression also supported this observation. Our results indicate that PPA enhances plant defenses against pathogen invasion through the plant redox system, and as a water-soluble compound that can promote plant growth, has broad potential applications in agriculture.
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影响因子：
7.2
作者：
Friedrich, L;Lawton, K;Ryals, J
通讯作者：
Ryals, J
影响因子：
7.4
作者：
Mishina, Tatiana E.;Zeier, Juergen
通讯作者：
Zeier, Juergen
DOI：
10.1073/pnas.1013020107
发表时间：
2010-10-12
影响因子：
11.1
作者：
Ford, Kevin A.;Casida, John E.;Wildermuth, Mary C.
通讯作者：
Wildermuth, Mary C.
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
7.4
作者：
Luna, Estrella;Bruce, Toby J. A.;Ton, Jurriaan
通讯作者：
Ton, Jurriaan