Noncoding RNA Surveillance: The Ends Justify the Means.
Noncoding RNA Surveillance: The Ends Justify the Means.
复制标题
DOI：
10.1021/acs.chemrev.7b00462
复制
发表时间：
2018-04-25
期刊：
影响因子：
62.1
通讯作者：
Wolin SL
中科院分区：
文献类型：
--
作者：
Belair C;Sim S;Wolin SL
许多监视途径通过降解不需要的、有缺陷的和潜在有害的非编码RNA（ncRNA）来塑造真核转录组。由于异常和过量的ncRNA在很大程度上被核糖核酸外切酶降解，因此这些RNA的关键特征是可接近的、无蛋白质的5 '或3'末端。大多数核糖核酸外切酶与辅因子一起起作用，所述辅因子识别具有可接近的5 '或3'末端的ncRNA和/或增加这些末端的可用性。非编码RNA监视途径首先在芽殖酵母中被描述，现在酵母途径的许多组分的高分辨率结构和对它们如何起作用的重要机制的理解。对人类细胞的研究揭示了这些途径与酵母对应物的相似和不同之处，也揭示了许多在芽殖酵母中缺乏等同物的途径。在这篇综述中，我们描述了酵母中发现的经过充分研究的途径和哺乳动物细胞研究中出现的新概念。我们还讨论了监视途径与瞬时保护新生ncRNA末端不受核糖核酸外切酶影响的伴侣蛋白竞争的方式，与在RNP内隔离这些末端的伴侣蛋白竞争的方式，以及与保护某些ncRNA末端不受核酸酶影响的末端修饰途径竞争的方式。
Numerous surveillance pathways sculpt eukaryotic transcriptomes by degrading unneeded, defective and potentially harmful noncoding RNAs (ncRNAs). Because aberrant and excess ncRNAs are largely degraded by exoribonucleases, a key characteristic of these RNAs is an accessible, protein-free 5’ or 3’ end. Most exoribonucleases function with co-factors that recognize ncRNAs with accessible 5’ or 3’ ends and/or increase the availability of these ends. Noncoding RNA surveillance pathways were first described in budding yeast, and there are now high-resolution structures of many components of the yeast pathways and significant mechanistic understanding as to how they function. Studies in human cells are revealing the ways in which these pathways both resemble and differ from their yeast counterparts, and are also uncovering numerous pathways that lack equivalents in budding yeast. In this review, we describe both the well-studied pathways uncovered in yeast and the new concepts that are emerging from studies in mammalian cells. We also discuss the ways in which surveillance pathways compete with chaperone proteins that transiently protect nascent ncRNA ends from exoribonucleases, with partner proteins that sequester these ends within RNPs, and with end modification pathways that protect the ends of some ncRNAs from nucleases.
登录
查看更多内容
影响因子：
11.4
作者：
Achsel, T;Brahms, H;Lührmann, R
通讯作者：
Lührmann, R
影响因子：
5.3
作者：
Ballarino, Monica;Pagano, Francesca;Bozzoni, Irene
通讯作者：
Bozzoni, Irene
影响因子：
1.5
作者：
Arhin, GK;Shen, SY;Ullu, E
通讯作者：
Ullu, E
影响因子：
10.5
作者：
Allmang, C;Petfalski, E;Mitchell, P
通讯作者：
Mitchell, P
影响因子：
56.9
作者：
Bertone, P;Stolc, V;Snyder, M
通讯作者：
Snyder, M