Hint1 expression inhibits proliferation and promotes radiosensitivity of human SGC7901 gastric cancer cells.
Hint1 expression inhibits proliferation and promotes radiosensitivity of human SGC7901 gastric cancer cells.
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Hint1表达抑制人SGC7901胃癌细胞增殖并促进放射敏感性
DOI：
10.3892/ol.2018.8900
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发表时间：
2018-08
期刊：
影响因子：
2.9
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Wei X;Zhou J;Hong L;Xu Z;Zhao H;Wu X;Chen J
胃癌是一种常见的恶性肿瘤，常常逃脱治疗。组氨酸三联体核苷酸结合蛋白1(Hint1)是一种单倍体缺陷的肿瘤抑制基因，它有助于细胞间的通讯，有助于调节细胞的增殖和生存，在胃癌中经常低表达。为了研究Hint1在胃癌中的作用，用小干扰RNA敲除人胃癌细胞系SGC-7901中Hint1的表达。结果表明，Hint1抑制了细胞增殖，减少了辐射诱导的DNA损伤修复，并导致G1期停滞，从而提高了胃癌细胞的放射敏感性。进一步的机制研究揭示了Hint1的一个新功能，即它作为细胞外信号调节激酶的负调节因子。这些结果证实了Hint1在人胃癌生物学中的重要作用。作为一种肿瘤生长抑制因子和放射增敏剂，该蛋白是一种潜在的生物标记物，并可能成为针对胃癌的特定治疗措施的一个有吸引力的靶点。
Gastric cancer is a prevalent, malignant tumor that frequently escapes treatment. Histidine triad nucleotide-binding protein 1 (Hint1) is a haploinsufficient tumor suppressor gene which contributes to intercellular communication, helps to regulate cell proliferation and survival, and is frequently underexpressed in gastric cancer. To examine the involvement of Hint1 in gastric cancer, small interfering RNA was used to knock down Hint1 expression in the human gastric cancer cell line SGC-7901. The data revealed that Hint1 inhibited cell proliferation, reduced radiation-induced DNA damage repair and caused G1 phase arrest, which increased the radiosensitivity of gastric cancer cells. Further mechanistic studies revealed a novel function of Hint1, whereby it acted as a negative regulator of extracellular signal-regulated kinase. These results demonstrated the critical function of Hint1 in the biology of human gastric cancer. Acting as a tumor growth suppressor and a radiosensitive agent, this protein is a potential biomarker and may be an attractive target for specific therapeutic interventions against gastric cancer.
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影响因子：
3.7
作者：
Liu H;Duan Z;Zheng H;Hu D;Li M;Tao Y;Bode AM;Dong Z;Cao Y
通讯作者：
Cao Y
影响因子：
7.4
作者：
Chuang, Jih-Ing;Huang, Jui-Yen;Ching, Cheng-Hsin
通讯作者：
Ching, Cheng-Hsin
DOI：
10.1073/pnas.1332160100
发表时间：
2003-06-24
影响因子：
11.1
作者：
Su, T;Suzui, M;Weinstein, IB
通讯作者：
Weinstein, IB
影响因子：
64.8
作者：
Lee, Yoonjin;Dominy, John E.;Choi, Yoon Jong;Jurczak, Michael;Tolliday, Nicola;Camporez, Joao Paulo;Chim, Helen;Lim, Ji-Hong;Ruan, Hai-Bin;Yang, Xiaoyong;Vazquez, Francisca;Sicinski, Piotr;Shulman, Gerald I.;Puigserver, Pere
通讯作者：
Puigserver, Pere
影响因子：
6.5
作者：
Kim, AL;Athar, M;Gautier, J
通讯作者：
Gautier, J