HIV-1 drug resistance and genetic diversity in a cohort of people with HIV-1 in Nigeria.
HIV-1 drug resistance and genetic diversity in a cohort of people with HIV-1 in Nigeria.
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DOI：
10.1097/qad.0000000000003098
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发表时间：
2022-01-01
期刊：
影响因子：
--
通讯作者：
Happi CT
中科院分区：
文献类型：
--
作者：
Oluniyi PE;Ajogbasile FV;Zhou S;Fred-Akintunwa I;Polyak CS;Ake JA;Tovanabutra S;Iroezindu M;Rolland M;Happi CT
这项研究的目的是提供关于尼日利亚HIV-1和耐药突变的遗传多样性的信息，因为对该国流行的变异的了解有限。我们使用先进的下一代测序平台Primer ID：研究高丰度和低丰度耐药突变的存在；表征抗逆转录病毒治疗（ART）经验丰富但妊娠初期个体中预先存在的整合酶链转移抑制剂（INSTI）突变；检测最近的HIV-1感染并表征HIV-1 （PWH）人群的亚型多样性。HIV-1亚型分析显示，CRF02_AG和G亚型在我们的研究人群中占主导地位。在检测灵敏度为30%丰度时，在3%的样品中鉴定出耐药突变（DRMs）。在10%的灵敏度水平下，27.3%的样本中识别出drm。我们没有发现任何与抗重力相关的主要INSTI突变。在我们的研究人群中仅检测到一例近期感染。我们的研究表明，在尼日利亚，含有杜鲁特韦的抗逆转录病毒治疗方案将是有效的。我们的研究还进一步强调了尼日利亚HIV-1的高遗传多样性，CRF02_AG和G亚型是尼日利亚HIV-1的主要循环形式。这两种传播形式的病毒在很大程度上推动了该国的流行病。
This study was designed to provide information on the genetic diversity of HIV-1 and drug resistance mutations in Nigeria, as there is limited understanding of variants circulating in the country. We used an advanced next-generation sequencing platform, Primer ID, to: investigate the presence of high and low abundance drug resistance mutations; characterize preexisting Integrase Strand Transfer Inhibitor (INSTI) mutations in antiretroviral therapy (ART)-experienced but dolutegravir-naive individuals; detect recent HIV-1 infections and characterize subtype diversity from a cohort of people with HIV-1 (PWH). HIV-1 subtype analysis revealed the predominance of CRF02_AG and subtype G in our study population. At detection sensitivity of 30% abundance, drug resistance mutations (DRMs) were identified in 3% of samples. At a sensitivity level of 10%, DRMs were identified in 27.3% of samples. We did not detect any major INSTI mutation associated with dolutegravir-resistance. Only one recent infection was detected in our study population. Our study suggests that dolutegravir-containing antiretroviral regimens will be effective in Nigeria. Our study also further emphasizes the high genetic diversity of HIV-1 in Nigeria and that CRF02_AG and subtype G are the dominant circulating forms of HIV-1 in Nigeria. These two circulating forms of the virus are largely driving the epidemic in the country.
影响因子：
3.7
作者：
Ajoge HO;Gordon ML;de Oliveira T;Green TN;Ibrahim S;Shittu OS;Olonitola SO;Ahmad AA;Ndung'u T
通讯作者：
Ndung'u T