Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development.
Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development.
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DOI：
10.1007/s12013-014-9982-8
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发表时间：
2014-11
影响因子：
2.6
通讯作者：
Gratton, Enrico
中科院分区：
文献类型：
--
作者：
Bonaventura, Gabriele;Barcellona, Maria Luisa;Golfetto, Ottavia;Nourse, Jamison L.;Flanagan, Lisa A.;Gratton, Enrico
我们描述了一种基于荧光寿命成像显微镜(FLIM)的方法来评估不同发育阶段(妊娠12天(E12)和16天(E16))神经细胞中各种膜的流动性。对于膜的测量，我们使用Laurdan探头，该探头通常用于利用光谱信息评估膜在模型和生物膜中的透水性。使用Flim方法，我们建立了一个基于不同脂类组成的模型系统校准的流动性标度。在神经元细胞中，我们发现内膜和质膜的流动性有显著差异，因为质膜的液体较少。然而，我们发现E12和E16两组细胞之间没有显著差异。与NIH3T3细胞的比较表明，E12和E16细胞的质膜明显比癌细胞的质膜更流畅。
We describe a method based on fluorescence lifetime imaging microscopy (FLIM) to assess the fluidity of various membranes in neuronal cells at different stage of development (day 12 (E12) and day 16 (E16) of gestation). For the FLIM measurements, we use the Laurdan probe which is commonly used to asses membrane water penetration in model and in biological membranes using spectral information. Using the FLIM approach we build a fluidity scale based on calibration with model systems of different lipid composition. In neuronal cells we found a marked difference in fluidity between the internal membranes and the plasma membrane, being the plasma membrane the less fluid. However, we found no significant differences between the two cells groups, E12 and E16. Comparison with NIH3T3 cells show that the plasma membranes of E12 and E16 cells are significantly more fluid than the plasma membrane of the cancer cells.
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影响因子：
3.8
作者：
Fereidouni, Farzad;Bader, Arjen N.;Gerritsen, Hans C.
通讯作者：
Gerritsen, Hans C.
影响因子：
2.5
作者：
HIRABAYASHI, Y;HIROTA, M;ANDO, S
通讯作者：
ANDO, S
影响因子：
4.8
作者：
Bourbon, NA;Yun, J;Kester, M
通讯作者：
Kester, M
影响因子：
2.9
作者：
Gallegos, Adalberto M.;Storey, Stephen M.;Ball, Judith M.
通讯作者：
Ball, Judith M.
影响因子：
2.7
作者：
De Vequi-Suplicy, Cintia C.;Benatti, Carlos R.;Lamy, M. Teresa
通讯作者：
Lamy, M. Teresa