The differential antiviral activities of chicken interferon α (ChIFN-α) and ChIFN-β are related to distinct interferon-stimulated gene expression.
The differential antiviral activities of chicken interferon α (ChIFN-α) and ChIFN-β are related to distinct interferon-stimulated gene expression.
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DOI：
10.1371/journal.pone.0059307
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Qu H;Yang L;Meng S;Xu L;Bi Y;Jia X;Li J;Sun L;Liu W
鸡α干扰素（ChIFN-α）和鸡β干扰素（ChIFN-β）均为Ⅰ型干扰素，是天然免疫系统中重要的抗病毒细胞因子。本研究利用病毒诱导鸡成纤维细胞DF-1表达ChIFN-α和ChIFN-β，并通过细胞病变抑制试验系统评价了重组ChIFN-α和ChIFN-β的抗病毒活性。结果表明，ChIFN-α对水泡性口炎病毒、纽卡斯尔病毒和禽流感病毒的抑制作用均强于ChIFN-β。为了阐明两种ChIFN之间差异抗病毒活性的机制，我们通过实时PCR测量IFN处理的DF-1细胞中IFN刺激基因（ISGs）的相对mRNA水平。ChIFN-α在编码抗病毒蛋白和MHC-I的几种ISG上显示出比ChIFN-β更强的诱导效力，而ChIFN-α在诱导参与信号传导途径的ISG方面不如ChIFN-β。总之，ChIFN-α和ChIFN-β对不同ISG组的诱导效力不同，ChIFN-α的抗病毒活性更强可能归因于下游抗病毒ISG的更高表达水平。
Chicken interferon α (ChIFN-α) and ChIFN-β are type I IFNs that are important antiviral cytokines in the innate immune system. In the present study, we identified the virus-induced expression of ChIFN-α and ChIFN-β in chicken fibroblast DF-1 cells and systematically evaluated the antiviral activities of recombinant ChIFN-α and ChIFN-β by cytopathic-effect (CPE) inhibition assays. We found that ChIFN-α exhibited stronger antiviral activity than ChIFN-β in terms of inhibiting the replication of vesicular stomatitis virus, Newcastle disease virus and avian influenza virus, respectively. To elucidate the mechanism of differential antiviral activities between the two ChIFNs, we measured the relative mRNA levels of IFN-stimulated genes (ISGs) in IFN-treated DF-1 cells by real-time PCR. ChIFN-α displayed greater induction potency than ChIFN-β on several ISGs encoding antiviral proteins and MHC-I, whereas ChIFN-α was less potent than ChIFN-β for inducing ISGs involved in signaling pathways. In conclusion, ChIFN-α and ChIFN-β presented differential induction potency on various sets of ISGs, and the stronger antiviral activity of ChIFN-α is likely attributed to the greater expression levels of downstream antiviral ISGs.
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