In vitro C3 deposition on Cryptococcus capsule occurs via multiple complement activation pathways.
In vitro C3 deposition on Cryptococcus capsule occurs via multiple complement activation pathways.
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DOI：
10.1016/j.molimm.2011.06.215
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发表时间：
2011-09
影响因子：
3.6
通讯作者：
Beenhouwer DO
中科院分区：
文献类型：
--
作者：
Mershon-Shier KL;Vasuthasawat A;Takahashi K;Morrison SL;Beenhouwer DO
Complement can be activated via three pathways: classical, alternative, and lectin. Cryptococcus gattii and C. neoformans are closely related fungal pathogens possessing a polysaccharide capsule composed mainly of glucuronoxylomannan (GXM), which serves as a site for complement activation and deposition of complement components. We determined C3 deposition on Cryptococcus spp. by flow cytometry and confocal microscopy after incubation with serum from C57BL/6J mice as well as mice deficient in complement components C4, C3, factor B, and mannose binding lectin (MBL). C. gattii and C. neoformans activate complement in EGTA-treated serum indicating that they can activate the alternative pathway. However, complement activation was seen with factor B−/− serum suggesting activation could also take place in the absence of a functional alternative pathway. Furthermore, we uncovered a role for C4 in the alternative pathway activation by Cryptococcus spp. We also identified an unexpected and complex role for MBL in complement activation by Cryptococcus spp. No complement activation occurred in the absence of MBL-A and -C proteins although activation took place when the lectin binding activity of MBL was disrupted by calcium chelation. In addition, alternative pathway activation by C. neoformans required both MBL-A and -C, while either MBL-A or -C was sufficient for alternative pathway activation by C. gattii. Thus, complement activation by Cryptococcus spp. can take place through multiple pathways and complement activation via the alternative pathway requires the presence of C4 and MBL proteins.
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影响因子：
3.1
作者：
KOZEL, TR;WILSON, MA;MURPHY, JW
通讯作者：
MURPHY, JW
影响因子：
15.9
作者：
Selander, B;Mårtensson, U;Sjöholm, AG
通讯作者：
Sjöholm, AG
影响因子：
5.8
作者：
Takahashi, K;Gordon, J;Ezekowitz, RAB
通讯作者：
Ezekowitz, RAB
影响因子：
3.1
作者：
SCHELENZ, S;MALHOTRA, R;BANCROFT, GJ
通讯作者：
BANCROFT, GJ
影响因子：
3.1
作者：
Shapiro, S;Beenhouwer, DO;Scharff, MD
通讯作者：
Scharff, MD