Gene transfer using bovine adeno-associated virus in the guinea pig cochlea.
Gene transfer using bovine adeno-associated virus in the guinea pig cochlea.
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作者：
将基因转移到耳蜗细胞中对于研究和治疗都是有用的。牛腺相关病毒（BAAV）是一种新型的病毒载体，具有长期基因表达的潜力，几乎没有副作用。在这项研究中，我们评估了转基因表达BAAV与β-肌动蛋白-GFP作为报告基因，在耳蜗的正常和中毒的豚鼠。我们使用两种不同的路线来覆盖耳蜗：中阶（SM）或鼓阶（ST）。在接种前、接种后7天和处死前即刻进行听觉脑干反应评估，以评估处理的功能结果。我们观察到由于手术侵入引起的阈值偏移，但没有与病毒相关的明显病理学。注射后14天，处死动物并对耳蜗进行组织学评估。落射荧光显示BAAV通过SM和ST接种转导了正常和感染动物的支持细胞。ST接种后膜迷路细胞中的转基因表达是一个重要的结果，因为这种途径对未来的临床应用具有更大的可行性。BAAV有助于有效转导膜迷路上皮细胞，致病性最低，可能成为临床上适用的内耳基因治疗。
Gene transfer into cells of the cochlea is useful for both research and therapy. Bovine adeno-associated virus (BAAV) is a novel viral vector with potential for long term gene expression with little or no side effects. In this study we assessed transgene expression using BAAV with ß-actin-GFP as a reporter gene, in cochleae of normal and deafened guinea pigs. We used two different routes to inoculate the cochlea: scala media (SM) or scala tympani (ST). Auditory brainstem response assessments were done prior to inoculation, 7 days after inoculation and immediately prior to sacrifice, to assess the functional consequences of the treatment. We observed threshold shifts due to the surgical invasion, but no apparent pathology associated with the virus. Fourteen days after the injection, animals were sacrificed and cochleae assessed histologically. Epi-fluorescence showed that BAAV transduced the supporting cells of both normal and deafened animals through SM and ST inoculations. Transgene expression in cells of the membranous labyrinth following ST inoculation is an important outcome because of the greater feasibility of this route for future clinical application. BAAV facilitates efficient transduction of the membranous labyrinth epithelium with minimum pathogenicity and may become clinically applicable for inner ear gene therapy.
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影响因子：
4.8
作者：
Coura Rdos S;Nardi NB
通讯作者：
Nardi NB
影响因子：
2.8
作者：
RAPHAEL, Y;ALTSCHULER, RA
通讯作者：
ALTSCHULER, RA
影响因子：
2.9
作者：
WEBSTER, M;WEBSTER, DB
通讯作者：
WEBSTER, DB
DOI：
10.1016/s0736-5748(96)00112-8
发表时间：
1997-07-01
影响因子：
1.8
作者：
Weiss, MA;Frisancho, JC;Raphael, Y
通讯作者：
Raphael, Y
影响因子：
4.2
作者：
Ladrech, Sabine;Wang, Jing;Lenoir, Marc
通讯作者：
Lenoir, Marc