Morphology, ultrastructure and molecular characterisation of Spiroxys japonica Morishita, 1926 (Spirurida: Gnathostomatidae) from Pelophylax nigromaculatus (Hallowell) (Amphibia: Ranidae)

Morphology, ultrastructure and molecular characterisation of Spiroxys japonica Morishita, 1926 (Spirurida: Gnathostomatidae) from Pelophylax nigromaculatus (Hallowell) (Amphibia: Ranidae)
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Spiroxys japonica Morishita 的形态学、超微结构和分子特征,1926 年(Spirurida:Gnathostomatidae)来自 Pelophylax nigromaculatus(Hallowell)(两栖类:Ranidae)

DOI:
10.1007/s00436-013-3720-9
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发表时间:
2014-03
影响因子:
2
通讯作者:
Zhang, Lu-Ping
Zhang, Lu-Ping
中科院分区:
医学3区
文献类型:
--
作者:
Xu, Zhen;Guo, Yan-Ning;Sato, Akiko;Zhang, Lu-Ping

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从黑斑侧褶蛙(Pelophylax nigromaculatus,哈洛威尔)体内采集到颚口线虫,经形态学鉴定为日本螺口线虫(Spiroxys japonica Morishita,1926)。光学和扫描电子显微镜被用来详细研究这个物种的形态。以前未报道的形态特征被揭示,其他的被纠正。此外,S.日本黑斑螺螺(Spiroxys hanzaki Hasegawa,Miyata & Doi,1998)和日本隐鳃螺(Spiroxys hanzaki)的第三期幼虫。日本收集的Lithobates catesbeianus(Shaw)(无尾目:蛙科),其特征在于使用分子方法,通过测序和分析核糖体[大核糖体DNA(18 S)和内部转录空间]和线粒体[细胞色素c氧化酶亚基1]的目标区域。新的形态学和遗传学数据有助于更准确地诊断这种迄今鲜为人知的线虫属。
Gnathostomatid nematodes identified morphologically as Spiroxys japonica Morishita, 1926 were collected from the dark-spotted frog Pelophylax nigromaculatus (Hallowell) (Amphibia: Ranidae) in China. Light and scanning electron microscopy were used to study the morphology of this species in detail. Previously unreported morphological features are revealed and others corrected. In addition, adult nematodes of S. japonica collected from P. nigromaculatus and Spiroxys hanzaki Hasegawa, Miyata & Doi, 1998 collected from Andrias japonicus (Temminck) (Caudata: Cryptobranchidae) in China and Japan, respectively, and the third-stage larva of S. japonica collected from Lithobates catesbeianus (Shaw) (Anura: Ranidae) in Japan, were characterised using molecular methods by sequencing and analysing ribosomal [large ribosomal DNA (18S) and internal transcribed space] and mitochondrial [cytochrome c oxidase subunit 1] target regions, respectively. The new morphological and genetic data contributes to a more accurate diagnosis of this hitherto little known nematode genus.
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