Nucleic acid testing for tuberculosis at the point-of-care in high-burden countries.
Nucleic acid testing for tuberculosis at the point-of-care in high-burden countries.
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DOI：
10.1586/erm.12.71
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发表时间：
2012-09
影响因子：
5.1
通讯作者：
Boyle DS
中科院分区：
文献类型：
--
作者：
Niemz A;Boyle DS
结核病的早期诊断有助于启动适当的治疗，并可限制这一高度传染性疾病的传播。然而，在缺乏必要资源的结核病流行国家，通常使用的结核病诊断方法速度慢，往往不敏感，繁琐，大多数患者无法使用。这篇综述讨论了核酸扩增技术，这是正在开发的快速近病人结核病诊断，是在市场上或正在进行临床评价。它们基于PCR或等温方法，并作为手动测定或部分/完全集成的仪器系统实施，由经过最低限度培训的人员在临床性能、成本、稳健性、质量保证和远程设置中的可用性之间进行相关权衡。在艾滋病毒阳性患者和儿科患者中，发现耐药结核病和诊断结核病的需求普遍未得到满足。
Early diagnosis of tuberculosis (TB) facilitates appropriate treatment initiation and can limit the spread of this highly contagious disease. However, commonly used TB diagnostic methods are slow, often insensitive, cumbersome and inaccessible to most patients in TB endemic countries that lack necessary resources. This review discusses nucleic acid amplification technologies, which are being developed for rapid near patient TB diagnosis, that are in the market or undergoing clinical evaluation. They are based on PCR or isothermal methods and are implemented as manual assays or partially/fully integrated instrument systems, with associated tradeoffs between clinical performance, cost, robustness, quality assurance and usability in remote settings by minimally trained personnel. Unmet needs prevail for the identification of drug-resistant TB and for TB diagnosis in HIV-positive and pediatric patients.
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影响因子：
3.5
作者：
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通讯作者：
Nkengasong, John N.
影响因子：
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作者：
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通讯作者：
Woods, GL
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9.4
作者：
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作者：
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通讯作者：
Alland, David
DOI：
10.1164/rccm.200709-1436oc
发表时间：
2008-04-01
影响因子：
24.7
作者：
Barnard, Marinus;Allbert, Heidi;Bosiman, Marlein E.
通讯作者：
Bosiman, Marlein E.